Base excision repair (BER) enzymes maintain the integrity of the genome, and in humans, BER mutations are associated with cancer. Given the remarkable sensitivity of DNA-mediated charge transport (CT) to mismatched and damaged base pairs, we have proposed that DNA repair glycosylases (EndoIII and MutY) containing a redox-active [4Fe4S] cluster could use DNA CT in signaling one another to search...

Practical and highly sensitive Sakalar quantification table of DNA (SQT-DNA) has been developed for the detection% of species-specific DNA amount in food products. Cycle threshold (Ct) data were obtained from multiple curves of real-time qPCR. The statistical analysis was done to estimate the concentration of standard dilutions. Amplicon concentrations versus each Ct value were assessed by the ...

Nickel phthalocyanine complex containing 4-(3,4-dicyanophenoxy)-3-methoxybenzoic acid group was synthesized and specified by way of FT-IR, NMR, UV/Vis procedures. The binding PcNi to CT-DNA examined via electronic absorption titration, emission melting temperature, viscosity measurement, agarose gel electrophoresis technics, respectively. DNA interaction activity against studied UV/Vi titration...

RecO is a recombination mediator protein (RMP) important for homologous recombination, replication repair and DNA annealing in bacteria. In all pathways, the single-stranded (ss) DNA binding protein, SSB, plays an inhibitory role by protecting ssDNA from annealing and recombinase binding. Conversely, SSB may stimulate each reaction through direct interaction with RecO. We present a crystal stru...

A new Schiff base ligands (H2L -H2L 3) was synthesized by the condensation reaction of 4-acyl pyrazolones and 2-benzothiazolyl thiosemicarbazide. The ruthenium(II) Schiff base complexes were prepared by the interaction of the ligands with [RuHCl(CO)(EPh3)2(B)] (E=P/As; B=PPh3/AsPh3/py). They were characterized by elemental analysis, IR, UV-vis, 1H, 13C, and 31P NMR spectral data. The interactio...

Effective adjuvants are needed to design effective vaccines against Chlamydia trachomatis (Ct). The aim of this study was to observe the immune response stimulated by inoculation of a DNA vaccine encoding a fusion protein comprising multiple Ct major outer membrane protein (MOMP) epitopes and human papillomavirus 6b L1 (HPV 6b L1) as the basis for designing a novel DNA vaccine against genital C...

Charge transport (CT) through DNA has been found to occur over long molecular distances in a reaction that is sensitive to intervening structure. The process has been described mechanistically as involving diffusive charge-hopping among low-energy guanine sites. Using a kinetically fast electron hole trap, N(4)-cyclopropylcytosine ((CP)C), here we show that hole migration must involve also the ...