A solution of polyhexamethylene biguanide hydrochloride (PHMB-HCl) was titrated with a standard solution of potassium poly(vinyl sulfate) (PVSK) using crystal violet (CV) as an photometric indicator cation. The end point was detected by a sharp absorbance change due to an abrupt decrease in the concentration of CV. A linear relationship between the concentration of PHMB-HCl and the end-point vo...

Single-molecule surface-enhanced Raman spectroscopy (SMSERS) of crystal violet (CV) has been reported since 1997, yet others have offered alternative explanations that do not necessarily imply SMSERS. Recently, the isotopologue approach, a statistically significant method to establish SMSERS, has been implemented for members of the rhodamine dye family. We provide the first demonstration of SMS...

The application of Leuco Crystal Violet (LCV) for the enhancement of footwear and fingerprint impression evidence was first utilized in the FBI Laboratory in late 1993, after learning from Mr. John F. Fischer (1) of the formulation of this reagent. Mr. Fischer subsequently made a presentation on this topic at the International Symposium on Footwear and Tire Tread Impression Evidence at the FBI ...

Effluent of various industries contain number of inhibitory substances which are continuously added in sea via river and results in water pollution. There is need to degrade the organic content to abate water pollution. In the present study, actinomycetes were isolated on glycerol aspargine agar from marine sample of Calangut beach, Goa. The isolates were tested for their potential to tolerate ...

We demonstrate observation of Raman signals of different analytes adsorbed on carbonaceous materials, such as, chemically reduced graphene, graphene oxide (GO), multi-walled carbon nanotube (MWCNT), graphite and activated carbon. The analytes selected for the study were Rhodamine 6G (R6G) (in resonant conditions), Rhodamine B (RB), Nile blue (NBA), Crystal Violet (CV) and acetaminophen (paracet...

Fluorescence correlation spectroscopy (FCS) is a standard tool for studying diffusion of molecules in solution, but is limited to low analyte concentrations, in the range between 10 pM and 1 nM. Such concentration limitations can be overcome by using a plasmonic nanoantenna which confines the electric field of excitation light into a tiny volume near its surface and thereby reduces the effectiv...

G-quadruplexes (GQs) are alternative DNA secondary structures that can form throughout the human genome and control the replication and transcription of important regulatory genes. Here, we established an ensemble fluorescence assay by employing two GQ-interacting compounds, N-methyl mesoporphyrin IX (NMM) and Crystal Violet (CV). This enables quantitative measurement of the GQ folding propensi...

The effect of oxidative stress exerted by 0.03% crystal violet, 0.3% bile salt, 4.0% NaCl and 8% ethanol on the survival and production of virulence factors among Acinetobacter haemolyticus isolates obtained from wastewater and river water samples was investigated. Though generally there was insignificant lethal effect against all the isolates, crystal violet exerted the highest lethal effect f...

Controlled shape and size with tuneable band gap (1.92-2.41 eV), nickel sulphide NPs was achieved in presence of thiourea or thioacetamide as sulphur sources with the variations of temperature and capping agents. Synthesized NPs were fully characterized by powder XRD, IR, UV-vis, DRS, FE-SEM, TEM, EDX, XPS, TGA and BET. Capping agent, temperature and sulphur sources have significant role in con...