In order to examine the prevalence of Cryptosporidium infection in wild rodents and insectivores of South Korea and to assess their potential role as a source of human cryptosporidiosis, a total of 199 wild rodents and insectivore specimens were collected from 10 regions of South Korea and screened for Cryptosporidium infection over a period of 2 years (2012-2013). A nested-PCR amplification of...

Accurate knowledge of the transport and deposition behavior for pathogenic Cryptosporidium parvum oocysts is needed to assess contamination and protect water resources. Experimental and modeling studies were undertaken to examine the roles of attachment, detachment, and straining on oocyst transport and retention. Saturated column studies were conducted using Ottawa aquifer sands (U.S. Silica, ...

The Cryptosporidium oocyst is encased in a robust wall that is extremely resistant to detrimental environmental factors such as chlorine used to disinfect potable water. Therefore, extracting oocyst DNA is not a trivial undertaking. Standard procedures used to extract DNA from oocysts, such as freeze–thaw (F/T) methods and DNA purification kits, are time-consuming and expensive and are difficul...

Epidemiologic and laboratory data suggest that coprodiagnostic methods may fail to detect Cryptosporidium oocysts in stool specimens of infected patients. To improve the efficacy of stool concentration procedures, we modified different steps of the Formalin-ethyl acetate (FEA) stool concentration technique and evaluated these modifications by examining stool samples seeded with known numbers of...

This study reports the first genetic characterisation of Cryptosporidium isolates in Brazil using real-time polymerase chain reaction (RT-PCR). A total of 1,197 faecal specimens from children and 10 specimens from human immunodeficiency virus-infected patients were collected between 1999-2010 and screened using microscopy. Forty-eight Cryptosporidium oocyst-positive isolates were identified and...

In order to improve genotyping and epidemiological analysis of Cryptosporidium spp., genomic data need to be generated directly from a broad range of clinical specimens. Utilizing a robust method that we developed for the purification and generation of amplified target DNA, we present its application for the successful isolation and whole-genome sequencing of 14 different Cryptosporidium homini...

background and objectives: cryptosporidiosis has not been reported as an endemic disease in kashmir, but high prevalence of cryptosporidium sp. has been found among asymptomatic (non-diarrheic) hiv positive immigrants in present study. due to increasing number of hiv positive immigrants in kashmir, cryptosporidium may become a public health problem in kashmir. materials and methods: a total of ...

Cryptosporidium is an obligate intracellular parasite that infects a wide variety of hosts. Twenty calves with cryptosporidiosis were divided into two groups. A single dose 2.5 mg/kg buparvaquone was administered intramuscularly to one group, while 100 mg/kg/day paromomycin orally the other group. The results obtained from study in which oocyst shedding and clinical parameters sucking reflex, m...

From December 1990 to June 1992 in Ahwaz, capital of Khoozestan province, 1333 patients referred to Khoozestan Parasitology Center, in 29 cases of whom Cryptosporidium pallium was detected. All infected patients were less than eight years of age. The parasite was most prevalent in the 0-1 year old age group. Though some C. parVlIl1l infected cases harboured Giardia lamblia, there was no rel...

AIMS To detect a wide range of Cryptosporidium species from human faeces by analysis of the Cryptosporidium oocyst wall protein gene by PCR. METHODS AND RESULTS The nested-assay comprised an initial amplification using a conventional thermocycler followed by real time PCR using a LightCycler with SYBR Green I for the characterization of the amplicons. The technique uses four sets of primers c...