The objective was to determine whether the hatching stage of cattle and swamp buffalo somatic cell nuclear-transferred (SCNT) blastocysts affected cryosurvival after vitrification, and whether addition of linoleic acid-albumin (LAA) to the IVC medium and Ficoll to the vitrification solution improves cryosurvival. Fused couplets were activated with ethanol and cycloheximide-cytochalasin D (day 0...

Background: Ovarian cryopreservation is an efficient way to preserve fertility of cancer patients before chemotherapy. This study was designed to evaluate the effect of apha lipoic acid (ALA) on reactive oxygen species (ROS) production and total antioxidant capacity (TAC) of cultured isolated pre-antral follicles derived from vitrified and non-vitrified ovarian tissue. Materials and Methods: Im...

background: the aim of this study was to investigate the effects of two vitrification protocols on mouse metaphase stage ii (mii) oocytes and evaluate their effects on the expression of heat shock protein a1a (hsp a1a) and mnsod genes. materials and methods: groups of approximately 15 oocytes without cumulus complexes were collected and vitrified with 10% (v/v) ethylene glycol (eg) + 10% (v/v) ...

Background: Controlled ovarian stimulation (COS) has been shown to advance endometrial maturation and affect adversely implantation in assisted reproduction technology (ART) cycles. It has been reported that there is a better embryo-endometrium synchrony in frozenthawed embryo transfer (FET) cycles than fresh embryo transfer (ET) cycles. The Background of this study was to compare ongoing pregn...

Objective Vitrification has been shown as one of the most effective methods of cryopreservation for mammalian embryos. However, there is no consensus which stage of embryonic development is the most appropriate for vitrification with subsequent maximal development after thawing. This study was carried out to explore and compare the effect(s) of vitrification on mouse 2-cell, 4-cell, 8-cell, mor...

Abstract Study question Can increased time in thaw solution improve oocyte survival and outcomes following vitrification/warming? Summary answer Exposing vitrified oocytes to the first step thawing for 90 seconds improved compared 60 second exposure. What is known already Oocytes are a sensitive cell type careful handling of cells required obtain high vitrification warming. Carefully timed expo...

background: the objective of this study was to investigate the effect of vitrification and in vitro maturation on the mitochondrial distribution and atp content of oocytes. methods: the oocytes at germinal vesicle (gv) and metaphase ii (mii) stages were recovered from 6-8 week old nmri strain female mice. the oocytes were divided into vitrified and non-vitrified groups. vitrification was done b...

Abstract Study question Do the oocytes retrieved after luteal phase ovarian stimulation (OS) have same survival rate as those follicular (OS)? Summary answer Oocyte vitrification is similar in a OS compared to phase. What known already Oocytes recently captured scientific interest dual gaining popularity. obtained added shorten time pregnancy per intention treat poor responders. Embryos with th...

1 Department of Biology, Faculty of Science, Kharazmi (Tarbiat moallem) University, Tehran, Iran. 2 Department of Biology, Faculty of Science, Kharazmi (Tarbiat moallem) University, Tehran, Iran. 3 Department of Community Medicine, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran. 4 Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran. 5 Cellular and Mo...