Cryopreservation is an important procedure in maintenance and clinical applications of mesenchymal stem/stromal cells (MSCs). Although the methods of cell freezing using various cryoprotectants are well developed and allow preserving structurally intact living cells, the freezing process can be considered as a severe cellular stress associated with ice formation, osmotic damage, cryoprotectants...

Spermatozoa of the freeze-tolerant wood frog (Rana sylvatica) were used to develop a general protocol for the frozen storage of amphibian spermatozoa. Tolerance of spermatozoa to cryoprotective agents and freezing in suspension (-80 degrees C) was determined from rates of sperm lysis and dual-fluorochrome vital dye assays. We tested the efficacy of four cryoprotectants (Me2SO, methanol, glycero...

Thylakoid membranes isolated from either spinach or chickpea leaves were used as a model system for evaluating the capacity of cyclitols to act as cryoprotectants. The effect of freezing for 3 h at -18 degrees C on cyclic photophosphorylation and electron transport was measured. The cyclitols, ononitol, O-methyl-muco-inositol, pinitol, quebrachitol and quercitol at 50-150 mol m(-3) decreased me...

Adding DE 18 maltodextrin (80 g kg(-1)) to high-fat minced mackerel was highly effective against lipid oxidation and protein and color changes during frozen storage. It increased the temperature of ice-melting onset (Tm') and decreased freeze concentration of solutes in the unfrozen water (UFW) phase, which would have allowed it to effectively slow such perturbations. This maltodextrin showed a...

Analysis of structural and functional parameters of HDL has gained significant momentum in recent years because they are stronger predictors of cardiovascular risk than HDL-cholesterol levels. Surprisingly, in most HDL studies, very low attention is paid to HDL storage, which might critically affect functional properties. In the present study, we systematically examined the impact of storage an...

The effects of four cryoprotectants (dimethylformamide (DMF), ethylene glycol (EG), glycerol and dimethyl sulfoxide (DMSO)) on monolayers phospholipids were investigated at high cryoprotectant concentration (10% v/v) relevant to cryoprotection, compared with previous work lower concentrations (5% v/v). results show that the interactions between cryoprotective agents (CPAs) lipids are complex, s...