Oral-Turinabol, dehydrochloromethyltestosterone (DHCMT), is a synthetic anabolic androgenic steroid (AAS) prohibited in sports by the World Anti-Doping Agency. We used accurate-mass measurements by liquid chromatography coupled to a quadrupole time-of-flight (LC/MS Q-TOF) system to investigate DHCMT metabolites. Nine potential biomarkers of Oral-Turinabol consumption, including glucuronide-conj...

Gold compounds are among the few therapeutic agents that can cause remission of aggressive rheumatoid arthritis. Although the mechanism for their toxicity and efficacy is unknown, gold levels in blood have been emphasized recently. In this report, we describe a systematic method to study the gold interaction with red blood cells and its distribution in the cell. Flow injection analysis (FIA) an...

A method for the identification and quantification of canonic and isoforms of phytochelatins (PCs) from Chlamydomonas reinhardtii was developed. After disulfide reduction with tris(2-carboxyethyl)phosphine (TCEP) PCs were derivatized with ferrocenecarboxylic acid (2-maleimidoyl)ethylamide (FMEA) in order to avoid oxidation of the free thiol functions during analysis. Liquid chromatography (LC) ...

Liquid chromatography coupled to electrospray ionisation high resolution mass spectrometry is an extremely powerful technique for both targeted and non-targeted analysis of organic aerosol.

Mulberry leaves have commonly been utilized in China as a herbal medicine for the treatment of diabetes for thousands of years. To evaluate the quality, an ultra-high performance liquid chromatography coupled with quadrupole time of flight mass spectrometry (UPLC-Q-TOF/MS) method was developed for identification of polyhydroxylated alkaloids with α-glucosidase inhibitor activity in mulberry lea...

Atazanavir is a commonly prescribed protease inhibitor for treatment of HIV-1 infection. Thus far, only limited data are available on the in vivo metabolism of the drug. Three systemic circulating metabolites have been reported, but their chemical structures have not been released publicly. Atazanavir metabolites may contribute to its effectiveness but also to its toxicity and interactions. Thu...

A new analytical method has been developed and is proposed for the rapid determination of eighteen common amino acids, including tryptophan, in plasma and dried blood spots, by liquid chromatography coupled with ionspray tandem mass spectrometry. Potentially the method can include other amino acids and can be used for the diagnosis of metabolic disease. The use of the ionspray tandem mass spect...