A method for osmicating, dehydrating, and flat-embedding large slabs of brain tissue in epoxy resin is presented. This permits the production of semithin sections for postembedding immunocytochemistry that are far larger than can be obtained with other embedding approaches. Vibratomed slabs, 50-200 microns thick and as large as 6 x 8 mm are embedded in a 'soft' Araldite epoxy. The slabs are lam...