For DNA purification to be functionally integrated into the microchip for high-throughput DNA analysis, a miniaturized purification process must be developed that can be easily adapted to the microchip format. In this study, we evaluate the effectiveness of a variety of silica resins for miniaturized DNA purification and gauge the potential usefulness for on-chip solid-phase extraction. A micro...

Traditional flavor analysis relies on gas chromatography coupled to mass spectrometry (GC-MS) methods. Here we describe an indirect method coupling volatile compound formation to an ARO9-promoter-LacZ reporter gene. The resulting β-galactosidase activity correlated well with headspace solid phase micro extraction (HS/SPME) GC-MS data, particularly with respect to the formation of rose flavor. T...

Solid phase extraction is one of the major applications of molecularly imprinted polymers fields for clean-up of environmental and biological samples namely molecularly imprinted solid-phase extraction. In this study, solid phase extraction using the imprinted polymer has been optimized with the experimental design approach for a triazine herbicide, named atrazine with regard to the critical fa...

We have a sample in front of us. It is of unknown composition, but we know that it is complex, containing anywhere from a few hundred to many thousand chemical components. It is of uncertain physical form perhaps it looks like a liquid, but it has solid particles floating in it. We think that a particular compound is present in that sample, somewhere and in some form, but we don't know how much...

Berrueta LA, Gallo B and Vicente F (1995) A review of solid-phase extraction: basic principles and new developments. Chromatographia 40(7/8): 474}483. Guzman NA, Park SS, Schaufelberger D et al. (1997) Review: new approaches in clinical chemistry: on-line analyte concentration and microreaction capillary electrophoresis for the determination of drugs, metabolic intermediates and biopolymers in ...

A new, sensitive and fast dispersive liquid-liquid microextraction (DLLME) coupled with micro-solid phase extraction (μ-SPE) was developed for determination of zearalenone (ZEN) in wheat samples. The DLLME was performed using acetonitrile/water (80:20 v/v) as the disperser solvent and 1-octanol as the extracting solvent.  The acetonitrile/water (80:20 v/v) solvent was also used to extract ZEN f...