A prospective, double-blind study was carried out to deter mine whether reactivity with concanavalin A (Con A) of human breast cancer cells was related to early disease recurrence. Mammary epithelial cells were isolated from 138 primary hu man breast cancers. The cells were placed in culture, and their reactivity with Con A was determined with a hemadsorption assay in which human erythrocytes t...

The relationshio between Con A-receptor mobility and Con A-induced agglutination of Novikoff hepatoma and normal rat liver cells was investigated. Novikoff cells, incubated with fluorescein-labelled Con A at 3 degrees C displayed uniform, ring-like surface fluorescence. Increasing the temperature of the cells to 37 degrees C caused capping of Con A receptors in approximately 65% of the cells, a...

Differences in adoption of selected practices used in welfare assessment and audit programs were contrasted among organic (ORG; n=192) herds and similarly sized conventional grazing herds (CON-GR; n=36), and conventional nongrazing herds (CON-NG; n=64). Criteria from 3 programs were assessed: American Humane Association Animal Welfare Standards for Dairy Cattle, Farmers Assuring Responsible Man...

The present study examines the effect of concanavalin A (Con A) on the blood insulin and glucose levels of rats. Male and female rats treated with Con A (62.5-500 micrograms/kg) for three days showed a dose- and time-dependent hyperinsulinemia that lasted more than 48 h. Male rats were more sensitive to Con A. Thus, 6 h after treatment with Con A the circulating insulin levels in male rats had ...

Binding of concanavalin A (con A) to the lymphocyte surface at room temperature leads to restriction of the mobility of a variety of cell surface receptors including those from immunoglobulin (Ig), O, H-2, beta2-microglobulin, Fc receptors results in "co-capping" of Ig, H-2, theta, Fc receptors, as well as receptors for other lectins. Addition of colchicine to the cell suspensions reverses this...

Lines of Chinese hamster ovary cells resistant to the lectins concanavalin A (Con A) and phytohemagglutinin-P (PHA-P) have been isolated and characterized. Lines were isolated by a stepwise, a single-step, or a cycling single-step procedure, from both mutagen-treated and untreated cultures. The resistant lines showed a higher efficiency of colony formation in the presence of the appropriate lec...

The effect of concanavalin A (Con A) on the capping of mouse lymphocyte surface immunoglobulin (surface lg), cross-linked by rabbit anti-mouse Ig antibody, and on the capping of mouse thymocyte 0 antigen, cross-linked by anti-0 alloantibody and rabbit anti-mouse lg antibody, has been studied by immunofluorescence, using fluorescein conjugated Con A and rhodamine-conjugated anti-mouse lg antibod...

La asociación con niveles altos de anticuerpos contra la enzima carboxilasa del ácido glutámico (anti-GAD) es bien conocida en el síndrome de la persona rígida o Stiff person syndrome (SPS) y, más recientemente, también se han descrito en un subgrupo de pacientes con ataxia cerebelosa1,2. Sobre la base del probable origen autoinmune de estos síndromes se han ensayado distintas terapias inmunomo...

posteriormente leveriracetam y gabapentina en el manejo del status epiléptico en pacientes con PAI. Sin embargo, ningún fármaco debiera ser descartado por completo si su uso resultase esencial. Con frecuencia tras el diagnóstico nos encontramos con fármacos considerados no seguros sin efectos adversos. No obstante, es importante recordar su posible interacción futura con otros fármacos que pudi...

Las empresas continuamente requieren de personal con una formación determinada y con una base de conocimientos necesarios para incorporarse a un proyecto. Es difícil obtener un equipo de trabajo con el mismo nivel de conocimientos; por tanto, es necesario contar con un departamento de capacitación que tenga como objetivo garantizar que los equipos de trabajo posean un mismo dominio sobre el áre...