When a difficult separation cannot be accomplished by changing the column and mobile phase conditions, two alternate methods are available. The most common approach is to increase the column length with identical packing material, and the second approach is to recycle the sample through the column. However, the former approach might lead to a limitation to the separation efficiency due to an in...

US Environmental Protection Agency Method 300.0 specifies the use of an IonPac AS4A anion-exchange column with a carbonate-hydrogencarbonate eluent and suppressed conductivity detection for the determination of inorganic anions in environmental waters by ion chromatography. Hydroxide eluents have not typically been used for the routine analysis of common inorganic anions due to the lack of an a...

The strategies employed in chromatography steps play a key role in downstream processes for monoclonal antibody (mAb) manufacture. This work addresses the integrated optimisation of chromatography step sequencing and column sizing in mAb purification processes. Chromatography sequencing decisions include the resin selection at each typical step, while the column sizing decisions include the num...

Supplement 1: Methods Purification procedure of tissue kallikrein Rat kallikrein was purified from urine to apparent homogeneity by ultrafiltration and ion-exchange chromatography on a DEAE-Sepharose FF column (GE Healthcare, Buckinghamshire, UK), affinity chromatography on an aprotinin (Sigma, MO, USA) coupled HiTrap NHS-activated Sepharose HP column (GE Healthcare) and gel filtration twice on...

antiviral proteins (avp), present in silkworm fecal matter, show activity against nuclear polyhedrosis virus (npv) in vitro and in vivo. the extract of silkworm fecal matter prepared in phosphate buffer solution of ph 7.5 was subjected to 50% solid ammonium sulfate precipitation to enrich avp, then which was dialyzed. the dialysate was applied to the column containing silica gel-g, the column e...

An extracellular xylanase was purified to homogeneity by sequential chromatography of Fomitopsis pinicola culture supernatants on a DEAE-sepharose column, a gel filtration column, and then on a MonoQ column with fast protein liquid chromatography. The relative molecular weight of F. pinicola xylanase was determined to be 58 kDa by sodium dodecylsulfate polyacrylamide gel electrophoresis and by ...

ethanolic crude extract of i. imbricata lindl. (iridaceae) was subjected to column chromatography on silica gel with varying portions of meoh: chcl3. nigicin (irisolone) was isolated and its identification carried out by ir, uv, ms, 1-d and 2-d nmr spectroscopy.

The example of column chromatography (Figure 1) demonstrates the typical features found in this analytical technique. The diagram shows an experiment where a two-component mixture is subjected to column chromatography. The column is packed with a solid material called the stationary phase. A liquid solvent or eluting solution is poured into the column and completely wets the solid packing mater...