نتایج جستجو برای: colony assay

تعداد نتایج: 273197  

Journal: :The Journal of clinical investigation 1981
E M Mazur R Hoffman E Bruno

We have recently described an assay system for human peripheral blood megakaryocyte colony-forming unit cells (CFU-M) using an anti-platelet glycoprotein antiserum probe to define megakaryocyte colonies grown in vitro. This system was applied to study the nature and regulation of human bone marrow CFU-M. In the absence of a specific megakaryocyte growth-promoting factor, 12.4 +/- 3.0 (means +/-...

2005
Richard K. Shadduck

These studies have evaluated a newly developed radioimmunoassay (RIA) for the measurement of colony-stimulating factor (CSF) in murine serum and other biologic fluids. The routine in vitro agar gel bioassay for CSF is influenced by high molecular weight serum lipoproteins and low molecular weight tissue-derived materials that are inhibitory to colony formation. Studies with normal serum reveale...

Journal: :Cold Spring Harbor protocols 2016
Lisa C Crowley Melinda E Christensen Nigel J Waterhouse

Measuring cell death with colorimetric or fluorimetric dyes such as trypan blue and propidium iodide (PI) can provide an accurate measure of the number of dead cells in a population at a specific time; however, these assays cannot be used to distinguish cells that are dying or marked for future death. In many cases it is essential to measure the proliferative capacity of treated cells to provid...

Journal: :Cancer research 1986
J P Eder R C Bast W P Peters D Henner E Sanchez S Schryber E Frei L E Schnipper

Autologous bone marrow transplantation allows the use of high dose chemotherapy by obviating dose limiting myelosuppression. The pharmacology of high dose chemotherapy has been inadequately explored, yet this information is critical to determine the timing of marrow infusion and assure that engraftment is not compromised. We have used the Salmonella mutagenesis test (SMT) and colony forming uni...

2015
Herbert Braselmann Agata Michna Julia Heß Kristian Unger

BACKGROUND Colony formation assay is the gold standard to determine cell reproductive death after treatment with ionizing radiation, applied for different cell lines or in combination with other treatment modalities. Associated linear-quadratic cell survival curves can be calculated with different methods. For easy code exchange and methodological standardisation among collaborating laboratorie...

Journal: :Journal of clinical microbiology 2008
Anna Lau Tania C Sorrell OkCha Lee Keith Stanley Catriona Halliday

We developed a multiplex tandem PCR (MT-PCR) assay for the rapid identification of 16 fungi directly from culture. MT-PCR results were concordant with phenotypic identification for all cultures studied (n = 183). The colony MT-PCR assay was rapid (<2 h), sensitive, and specific in identifying fungal pathogens directly from primary isolation plates.

Journal: :Journal of clinical microbiology 2016
A R Arnold C-A D Burnham B A Ford S D Lawhon S K McAllister D Lonsway V Albrecht R C Jerris J K Rasheed B Limbago E M Burd L F Westblade

The performance of a rapid penicillin-binding protein 2a (PBP2a) detection assay, the Alere PBP2a culture colony test, was evaluated for identification of PBP2a-mediated beta-lactam resistance in human and animal clinical isolates of Staphylococcus intermedius group, Staphylococcus lugdunensis, and Staphylococcus schleiferi. The assay was sensitive and specific, with all PBP2a-negative and PBP2...

Journal: :iranian journal of pediatric hematology and oncology 0
f skandari department of hematology and blood banking, faculty of medical science, tarbiat modares university, tehran, iran a allahverdi department of hematology and blood banking, faculty of medical science, tarbiat modares university, tehran, iranسازمان اصلی تایید شده: دانشگاه تربیت مدرس (tarbiat modares university) h nasiri hematology-oncology and stem cell transplantation research center, tehran university of medical science, tehran, iranسازمان اصلی تایید شده: دانشگاه تربیت مدرس (tarbiat modares university) m azad department of medical laboratory sciences, faculty of allied medicine, qazvin university of medical science, qazvin, iraسازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (tehran university of medical sciences) n kalantari department of hematology and blood banking, faculty of medical science, tarbiat modares university, tehran, iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی قزوین (qazvin university of medical sciences) m soleimani department of hematology and blood banking, faculty of medical science, tarbiat modares university, tehran, iranسازمان اصلی تایید شده: دانشگاه تربیت مدرس (tarbiat modares university)

background the aim of this study was the ex vivo expansion of umbilical cord blood hematopoietic stem cells on biocompatible nanofiber scaffolds. materials and methods cd133+ hematopoietic stem cells were separated from umbilical cord blood using midimacs (positive selection) system by means of monocolonal antibody cd133 (microbeads) subsequently, flowcytometry method was done to assess the pur...

Journal: :Blood 1988
K M Zsebo V N Yuschenkoff S Schiffer D Chang E McCall C A Dinarello M A Brown B Altrock G C Bagby

Cultured mononuclear phagocytes produce soluble factors that stimulate endothelial cells to release GM-colony-stimulating activity (GM-CSA). One such factor was recently identified as interleukin 1 (IL 1). Studies were designed to determine which types of granulopoietic factors are released by IL 1-stimulated endothelial cells. Supernatants from endothelial cells cultured for 3 days in medium c...

Journal: :Nihon Jibiinkoka Gakkai kaiho 1991
M Ohnishi R Pawankar H Otsuka M Ikeda T Fujikura M Okuda

We examined the conditions, kinetics and cell sources for basophil/mast cell and eosinophil colony stimulating activity (CSA) production by nasal epithelial cells taken from house dust (HD) nasal allergic patients. Epithelial scrapings removed from HD nasal allergic patients were grown to confluence over 7 days as a monolayer of epithelial cells in medium supplemented with fetal calf serum (FCS...

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