of bloody diarrhea. Except for partial gastroduodenectomy because of a duodenal ulcer, the subject was healthy and was not taking any regular medication. He had not recently visited any foreign countries. Physical examination was unremarkable. Routine laboratory tests and complete blood analysis were normal. Total colonoscopy was performed after standard bowel preparation, without sedation. Col...

BACKGROUND Cytochrome P450 (CYP) enzymes in epithelial cells lining the alimentary tract play an important role in both the elimination and activation of (pro-)carcinogens. To estimate the role of cytochrome P450 in carcinogenesis of the colon, expression patterns and protein levels of four representative CYPs (CYP2C, CYP2E1, CYP3A4 and CYP3A5) were determined in colon mucosa of normal and aden...

Carbohydrate compositions of the membrane and cytoplasmic fractions of human normal and cancerous colonic mucosa were compared in patients with blood groups O and B. The total sugar content in both fractions was reduced in the cancer tissues to about one-third of that in the normal colonic mucosa. The sugars that are associated with mucinous glycoproteins such as fucose and N-acetylgalactosamin...

AIM To investigate intestinal alkaline phosphatase (iAP) in the intestinal mucosa of children with inflammatory bowel disease (IBD). METHODS Colonic biopsy samples were taken from 15 newly diagnosed IBD patients and from 10 healthy controls. In IBD patients, specimens were obtained both from inflamed and non-inflamed areas. The iAP mRNA and protein expression was determined by reverse transcr...

A sensitive 4 h 51Cr-release cytotoxicity assay has been developed using as targets colonic epithelial cells obtained by Dispase-collagenase digestion of resected mucosa or colonoscopic biopsies. Peripheral blood mononuclear cells (MNC) from most healthy donors showed low, but significant levels of cytotoxicity for normal epithelial cell target cells of 8.7 (4.4) % (mean (SD] and similar levels...

Bile acids are increasingly discussed in the genesis of colorectal carcinoma. In a pilot study we analysed bile acids in tumor tissue, tumor-free mucosa, serum, and feces o £ 6 patients with recta1 and 5 patients with colonic carcinoma, 5 patients dead from nongastroenterological diseases and two operated on for benign colonic stenosis seryed as controis for celorecta1 mucosa, 16 healthy person...

Many colonic mucosal genes that are highly regulated by microbial signals are differentially expressed along the rostral-caudal axis. This would suggest that differences in regional microbiota exist, particularly mucosa-associated microbes that are less likely to be transient. We therefore explored this possibility by examining the bacterial populations associated with the normal proximal and d...

Transitional mucosa of the colon is the tissue immediately adjacent to a cancer, which has histochemical and ultrastructural features similar to those seen in neoplasia. Therefore, it has been hypothesized in the past that this tissue represents the premalignant antecedent to colonic adenocarcinoma. Other investigators have disputed this explanation because similar changes are found adjacent to...