Recessive dystrophic epidermolysis bullosa (RDEB) ~ is a debilitating and often fatal disease characterized by repeated blistering after minor trauma. The morphologic finding of collagen degeneration (1) coupled with increased collagenase activity in explant cultures of skin from RDEB patients (2, 3) suggested that excessive collagenase might be repsonsible for the blistering phenomenon. This p...

Testicular macrophages (TMs) are located in the interstitial tissue of male gonad. These phagocytic cells take part in forming the organ-specific functional blood-testis barrier and participate in the regulation of the local hormonal balance. In the present study, we isolated TMs from testicular tissues using previously described methods--mechanical (M-TMs) or enzymatic, by treatment with colla...

Tissue inhibitor of metalloproteinases (TIMP) and collagenase inhibitory activity were measured in the sputum from nine subjects with chronic bronchitis before and five days after treatment with corticosteroid (oral prednisolone, 40 mg daily). The mean sputum TIMP concentrations for eight of the nine subjects increased from 3.1 (SD 0.87) micrograms/ml to 5.8 (1.9) micrograms/ml (2p less than 0....

Remodeling of fibrillar collagen in mouse tissues has been widely attributed to the activity of collagenase-3 (matrix metalloproteinase-13 (MMP-13)), the main collagenase identified in this species. This proposal has been largely based on the repeatedly unproductive attempts to detect the presence in murine tissues of interstitial collagenase (MMP-1), a major collagenase in many species, includ...

Purified human platelets were found to contain a collagenase inhibitor that is immunologically, functionally, and chromatographically identical to that produced by human skin fibroblasts. None of the other formed elements of the blood (erythrocytes, granulocytes, mononuclear cells) possessed detectable quantities of this protein. Virtually all the collagenase inhibitor contained within platelet...

The ability of cellular vascular components including endothelial cells, smooth muscle cells, and fibroblasts to interact with the collagenolytic activity of invasive human tumor cell lines has been investigated. The human HT1080 fibrosarcoma and Bowe melanoma cells, which rapidly digest collagenous proteins in vitro, failed to dissolve them when cocultivated with bovine endothelial cells. This...

A basal promoter for the M, 72,000 type IV collagenase gene was specifically defined by chloramphenicol acetyltransferase assays of a nested set of 5' upstream fragments containing the promoter region. This core promoter is TACATCT and is a noncanonical TATA box that fits the TATA consensus sequence. This sequence begins 26 base pairs in the upstream direction from the start site of transcripti...

Objective: Collagen is one of the main components in connective tissue (CT) and contributes to background toughness beef. It known that living animals, collagen can be degraded remodeled by collagenase matrix metalloproteinases (MMP); however, it unclear if MMP impact CT texture during postmortem aging Therefore, this study aimed understand how activity may degradation beef three different cuts...

BACKGROUND/AIMS A recent decrease in patient survival has been reported among hepatitis C virus (HCV)-infected liver transplant recipients and this may be attributable to progression of fibrosis. We reported previously that cyclosporine suppressed the proliferation of, and collagen production in, hepatic stellate cells (HSCs). Here, we investigated the effects of NIM811, a cyclosporine analogue...

Bone resorption is an important aspect of chronic otitis media contributing to many complications of this disease. It is postulated that the mechanism of this localized destructive process is chemical in origin. Collagenase, lysosomal enzymes, prostaglandins, and other cell mediators are thought to induce bone resorption, but the site of action and cellular origin of these substances remains un...