Objective: Blood culture (BC) is gold standard for diagnosis of fungemia. Contamination BC a major problem worldwide, since it may delay actual diagnosis. The aim this study was to evaluate “contaminant” vials with prolonged incubation (max 30 days) antimicrobial supplemented media observe any mycological growth. Materials and Methods: Routine BCs optained patients XXX Hospital year period were...

Seeds sterilized with sodium hypochlorite (NaOCl) retained sufficient amounts to interfere with studies of amino acid metabolism of the sterilized seeds during germination. Repeated washing in water did not remove NaOCl completely. However, soaking the seeds for 10 min in 0.01 n HCl removed NaOCl completely, without reducing germinability.Residual NaOCl reacted with the amino acids and reduced ...

This study aims to investigate the effect of Bifidobacterium adolescentis against noroviruses (NoVs). Murine norovirus-1 (MNV-1) used as a surrogate was detected by plaque assay and RT-qPCR. Human NoV virus like particles (VLPs) were detected by cell-binding assay. It was shown that the presence of B. adolescentis could inhibit the multiplication of MNV-1 on RAW 264.7 cells within 48 h of co-in...

The effects of TGF-β on dendritic cells (DCs) on the tumor microenvironment are not well understood. We report, here, the establishment of an in vitro lung cancer microenvironment by co-incubation of seminaphtharhodafluor (SNARF) labeled Lewis lung cancer (LLC) cells, carboxyfluorescein succinimidyl ester (CFSE) labeled fibroblasts and 4-chloromet...

Methanogens and sulphate reducing bacteria compete for H2 in the human colon, and, as a result, faeces usually contain high concentrations of just one of these two organisms. There is controversy over which of these organisms wins the competition for H2, although theoretical data suggest that sulphate reducing bacteria should predominate. To elucidate this question experiments were undertaken i...

Treponema pallidum (Nichols virulent) was incubated with and without cells in cell culture medium reduced to -275 mV Ecal, pH 7.3, under deoxygenated conditions. Five to ten percent of the treponemes attached to cells and remained motile for at least 120 h in cell-treponeme systems of co-incubation. Virulent treponemes could be detected after 120 to 144 h in the supernatant fluids of cell-trepo...

In presence of 7.5 microM of curcumin, no embryos or larva of zebrafish survived 3 days of incubation; however, coincubation with 144 microg/ml silymarin increased the survival rates of curcumin-treated embryos and larvae to about 70%. Moreover, in presence of 12.5 microM curcumin, all embryos died after 2 days of incubation; however, co-treatment with 144 microg/ml silymarin increased the surv...