PURPOSE The purpose of this study was to evaluate the relationship between apoptotic activity and clonogenic radiosensitivity in vitro using an insulin-like growth factor I receptor (IGF-IR) signaling model, which is known to exert tumorigenic and antiapoptotic effects. EXPERIMENTAL DESIGN We used mouse embryo fibroblast cell lines expressing either human IGF-IR [R+(Wt) and R+] or the marker ...

Pancreatic cancer (PC) is one of the most aggressive human malignancies worldwide and is the fourth leading cause of cancer-related deaths. Curcumin (diferuloylmethane) is a polyphenol derived from the Curcuma longa plant. Certain studies have demonstrated that curcumin exerts its anti-tumor function in a variety of human cancers including PC, via targeting multiple therapeutically important ca...

One-third of patients with medulloblastoma die due to recurrence after various treatments including radiotherapy. Although it has been postulated that cancer stem-like cells are radio-resistant and play an important role in tumor recurrence, the "stemness" of medulloblastoma cells surviving irradiation has not yet been elucidated. Using a medulloblastoma cell line ONS-76, cells that survived ga...

PURPOSE Breast cancer is an important cause of death among women. The development of radioresistance in breast cancer leads to recurrence after radiotherapy. Caffeic acid phenethyl ester (CAPE), a polyphenolic compound of honeybee propolis, is known to have anticancer properties. In this study, we examined whether CAPE enhanced the radiation sensitivity of MDA-MB-231 (estrogen receptor-negative...

BACKGROUND The protein kinase CK2 sustains multiple pro-survival functions in cellular DNA damage response and its level is tightly regulated in normal cells but elevated in cancers. Because CK2 is thus considered as potential therapeutic target, DNA double-strand break (DSB) formation and rejoining, apoptosis induction and clonogenic survival was assessed in irradiated mammalian cells upon che...

Liver cancer has a poor prognosis predominantly resulting from tumour heterogeneity and limited treatment options. The two predominant types of liver are Hepatocellular carcinoma (HCC) Intrahepatic Cholangiocarcinoma (iCCA). CD44v6, splice variant the hyaluronic acid receptor CD44, is marker for stem cells, known to impact formation metastasis in several cancers. expression CD44v6 dramatically ...

Purpose: To investigate the effect of excisanin A on human hepatocellular carcinoma cells as well to elucidate its mechanism action.
 Methods: Molecular docking was used determine binding characteristics HIF-1α protein. The transcriptional activation and viability were assessed using Luciferase reporter MTT assay. protein in nucleus assayed western blot immunofluorescence. VEGF mRNA levels...

An efficient and rapid cytotoxicity assay has been developed, particularly for radiobiological studies, utilizing 96-well microtiter plates. Several days after treatment, cell numbers per well were measured by fluorescent intensity using an automatic reader after staining with the DNA specific dye Hoechst 33258. For radiobiological applications, a microtiter plate irradiation box was designed a...