RATIONALE Bunyaviruses have become a major threat to both humans and livestock in Europe and the Americas. The nucleocapsid (N) protein of these viruses is key to the replication cycle and knowledge of the N oligomerisation state is central to understanding the viral lifecycle and for development of therapeutic strategies. METHODS Bunyamwera virus and Schmallenberg virus N proteins (BUNV-N an...

Rhp14 of Schizosaccharomyces pombe is homologous to human XPA and Saccharomyces cerevisiae Rad14, which act in nucleotide excision repair of DNA damages induced by ultraviolet light and chemical agents. Cells with disrupted rhp14 were highly sensitive to ultraviolet light, and epistasis analysis with swi10 (nucleotide excision repair) and rad2 (Uve1-dependent ultraviolet light damage repair pat...

Leptospirosis is a worldwide zoonosis caused by pathogenic Leptospira. The whole-genome sequence of L. interrogans serovar Copenhageni together with bioinformatics tools represent a great opportunity to search for novel antigen candidates that could be used as subunit vaccine against leptospirosis. We focused on six genes encoding for conserved hypothetical proteins predicted to be exported to ...

Intracellular delivery of functional macromolecules using peptide transduction domains (PTDs) is an exciting technology with both experimental and therapeutic applications. Recent data indicate that PTD-mediated transduction occurs via fluid-phase macropinocytosis involving an intracellular pH drop to approximately 5. Nitrilotriacetic acid (NTA)-coordinated metals avidly bind hexahistidine-tagg...

The article analyzes a wide range of philosophical and anthropological subjects in the works French philosopher Paul Ricoeur. Rejecting idea system-creation, Ricoeur creates generalized image person form polemical, sometimes marginal notes relation to other European thinkers. His reveal an original view on problems human subjectivity, Ego, personality, selfness, identity, etc. author shows that...

Sam Shepard is one of the foremost living American Playwrights. He has always been concerned with the problem of identity, specifically the fragmented identity of man living in the postmodern era. Such characterization is evident in his plays: True West, Fool for Love, and A Lie of the Mind. Thus, the present article is to demonstrate the fragmented identity of Lee and Austin, Eddie and May, an...

This paper presents a systemic-constructivist approach to working therapeutically with couples. Therapeutic change is optimal when both the intrapersonal processes of each individual and their unique interpersonal system are synthesized. This approach targets partners’ ways of knowing and experiencing themselves in the context of their relationship. The therapist endeavors to engage the aspect ...

This paper examines the changing methods, underlying motives, clienteles and controversy surrounding posthumous commemorations of Lord Lister in Britain. The importance of the commemorations for professional identity formation continues throughout the twentieth century, but World War I appears as a turning point. The constituencies commemorating Lister change from broadly international, nationa...