The environment of high-redshift galaxies is characterized by both wind-driven outflowing gas and gravitationally infalling streams. To investigate such galaxy-IGM interplay we have generated synthetic optical absorption line spectra piercing the volume surrounding a starbursting analog of a Lyman Break Galaxy selected in a z ≈ 3 output from a SPH simulation, including a detailed treatment of m...

Large scale production of economically viable biofuels can only be achieved from widely available resources, notably lignocellulosic biomass. This feedstock is largely composed by complex carbohydrates, which can be enzymatically hydrolyzed and converted to fuels and chemicals by fermentative organisms. Lignocellulosic biomass is recalcitrant to enzymatic degradation and therefore, a pretreatme...

Nitro-oleic acid (OA-NO2), acting as anti-inflammatory signaling mediators, are involved in multiple signaling pathways. Lipoprotein-associated phospholipase A2 (Lp-PLA2) is well known as a cardiovascular risk biomarker. Our results showed that OA-NO2 downregulated the expression of Lp-PLA2 in a time- and dose-dependent manner, whereas native OA had no such effect. Furthermore, OA-NO2 could rep...

Plant organisms contain a large number of genes belonging to numerous multigenic families whose evolution size reflects some functional constraints. Sequences from eight multigenic families, involved in biotic and abiotic responses, have been analyzed in Eucalyptus grandis and compared with Arabidopsis thaliana. Two transcription factor families APETALA 2 (AP2)/ethylene responsive factor and GR...

The Int protein specified by bacteriophage lambda is required for the recombination event that integrates the viral DNA into the host genome at its specific attachment site. Using a DNA-binding assay, we have partially purified the Int protein and studied some of the features of its binding specificity and regulation. The DNA-binding activity is attributed to Int protein because the activity is...

Infrared spectra for the carbon monoxide complex with myoglobin isolated as the oxygenyl species from bovine heart muscle were carefully examined in the C--O stretch region as either the pH or the temperature was varied. Deconvolutions of these spectra into bands of Gaussian shape suggest the presence of four bands near 1938(I), 1944(II), 1954(III), and 1965(IV) cm-1 with halfband widths of abo...

A new immunoaffinity technique for purifying anti-apolipoprotein CIII antibodies has been developed by isolating bands of apo CIII2 from isoelectric focusing (IEF) gel. Total apo C was obtained from delipidated very-low-density lipoprotein by chromatography on Sephacryl S 200. Apo CIII2 was separated from the apo CII band by IEF on polyacrylamide gel with use of pH 4-6 ampholytes. After sonicat...

Apolipoprotein-CIII (apoCIII) is an abundant blood glycoprotein associated with lipoprotein particles. Three different glycoforms have been described, all containing a mucin-type core-1 O-glycosylation with either zero, one or two sialic acids. Changes in the relative abundance of these glycoforms have been observed in a variety of different pathologies. In this study, ultrahigh resolution 15T ...