Lignin degradation by the white rot basidiomycete Phanerochaete chrysosporium involves various extracellular oxidative enzymes, including lignin peroxidase, manganese peroxidase, and a peroxide-generating enzyme, glyoxal oxidase. Recent studies have suggested that laccases also may be produced by this fungus, but these conclusions have been controversial. We identified four sequences related to...

The trans-dihydrodiols produced during the metabolism of phenanthrene by Cunninghamella elegans, Syncephalastrum racemosum, and Phanerochaete chrysosporium were purified by high-performance liquid chromatography (HPLC). The enantiomeric compositions and optical purities of the trans-dihydrodiols were determined to compare interspecific differences in the regio- and stereoselectivity of the fung...

Two methods were used to compare the biodegradation of six polychlorinated biphenyl (PCB) congeners by 12 white rot fungi. Four fungi were found to be more active than Phanerochaete chrysosporium ATCC 24725. Biodegradation of the following congeners was monitored by gas chromatography: 2,3-dichlorobiphenyl, 4,4'-dichlorobiphenyl, 2,4',5-trichlorobiphenyl (2,4',5-TCB), 2,2',4,4'-tetrachlorobiphe...

Olive mill wastewater (OMW) constitutes a major cause of environmental pollution in olive-oil producing regions. Sixty wood-rot macrofungi assigned in 43 species were evaluated for their efficacy to colonize solidified OMW media at initially established optimal growth temperatures. Subsequently eight strains of the following species were qualified: Abortiporus biennis, Ganoderma carnosum, Hapal...

Of the five species of white rot fungi evaluated for their ability to decolorize Amaranth, Remazol Black B, Remazol Orange, Remazol Brilliant Blue, Reactive Blue, and Tropaeolin O in agar plates, Bjerkandera sp. BOS55, Phanerochaete chrysosporium, and Trametes versicolor displayed the greatest extent of decoloration. In static aqueous culture, the three cultures formed fungal mats which did not...

Lignocellulose degradation and activities related to lignin degradation were studied in the solid-state fermentation of cotton stalks by comparing two white rot fungi, Pleurotus ostreatus and Phanerochaete chrysosporium. P. chrysosporium grew vigorously, resulting in rapid, nonselective degradation of 55% of the organic components of the cotton stalks within 15 days. In contrast, P. ostreatus g...

AMBER VANDEN WYMELENBERG, BERNARD JANSE, JILL GASKELL, DIANE DIETRICH, MARCELO VALLIM & DAN CULLEN*. Institute for Microbial and Biochemical Technology, U.S. Department of Agriculture, Forest Service, Forest Products Laboratory, Madison, WI 53705. Department of Bacteriology, University of Wisconsin, Madison, WI 537062, Department of Microbiology, University of Stellenbosch, Stellenbosch, South ...

Phanerochaete chrysosporium immobilized on different support materials, such as polyurethane foam (PUF) and scouring web (SW), in shake cultures, was able to decolourize efficiently the sugar refinery effluent in a long-term repeated-batch operation. The decolourization medium composition was optimized using PUF-immobilized fungus. Addition of glucose was obligatory and the minimum glucose conc...

A total of 95 isolates, belonging to 33 species of five dermatophyte genera, i.e. Arthroderma (15 species), Chrysosporium (two), Epidermophyton (one), Microsporum (three) and Trichophyton (12), were studied using internal transcribed spacer 2 (ITS2)-PCR-RFLP analysis (ITS2-RFLP), consisting of amplification of the ITS2 region, restriction digestion with BstUI (CG/CG) and restriction fragment le...

The abilities of whole cultures of Phanerochaete chrysosporium and P. chrysosporium manganese peroxidase-mediated lipid peroxidation reactions to degrade the polycyclic aromatic hydrocarbons (PAHs) found in creosote were studied. The disappearance of 12 three- to six-ring PAHs occurred in both systems. Both in vivo and in vitro, the disappearance of all PAHs was found to be very strongly correl...