High-resolution anion-exchange chromatography of human urine can resolve more than 150 constituents that absorb ultraviolet light. Prefractionation of urine on Sephadex G.10 helped us identify the constituents and simplified the anion-exchange chromatogram. The material associated with the 13 peaks obtained by gel chromatography was pooled and concentrated into six fractions, which were subsequ...

A rapid determination of decabromodiphenyl ether (DeBDE) in polystyrene (PS) by thermal desorption (TD)-GC/MS was studied. The TD-GC/MS method using a pyrolysis-GC/MS system allowed the quick quantification of DeBDE in a waste TV back plate composing of a PS flame-retarded with polybrominated diphenyl ethers on the basis of the resulting chromatogram with a ca. 4% relative standard deviation wi...

Preparations from the parotid gland venom of the toad Bufo marinus have been analyzed by paper chromatography for the presence of adrenalin precursors. In addition to adrenalin itself, hydroxytyramine was identified and a second compound which appeared to be an adrenalin ester-perhaps lactyl-adrenalin. Two other compounds which appeared on the chromatogram could not be identified. Neither norad...

A blood sample from a 39-year-old female patient with newly diagnosed type 2 diabetes was assayed for hemoglobin (Hb) A1c on a Bio-Rad D10 HPLC platform. No peak for A1c was seen on the chromatogram (Fig. 1A), and the result was reported as 0%. A complete blood count was clinically significant for an Hb concentration of 10.9 g/dL (reference interval 12–15 g/dL) and a mean corpuscular volume of ...

Synthesis of indole derivatives was investigated and compared to the reaction of phenylhydrazine and ketones in the presence of the heterogeneous catalysts like kaolinite, montmorillonite K10 and Fe3O4 MNPs in ethanol under reflux conditions. After comparing the HPLC chromatogram of products it was compared and found that kaolin and montmorillonite K10 are better and more efficient candidate th...

Justicia adhatoda L. which belongs to the family of Acanthaceae contains alkaloids like vasicine, vasicinone and deoxyvasicine. These alkaloids give the plant its expectorant activity, antispasmodic, antiseptic and antihelmintic properties. Hence, in the present investigation attempts were made to isolate and characterise alkaloids from callus and suspension cultures of J. adhatoda. Alkaloids w...

In this study, the bioactive compounds of Vernonia cinerea have been evaluvated using GC-MS. The chemical compositions of the whole plant methanol extract of Vernonia cinerea were investigated using Perkin-Elmer Gas Chromatography Mass Spectroscopy. GC-MS analysis of V. cinerea plant methanol extract revealed the existence of the GC-MS chromatogram of the seven peaks presented .The major compou...

The PFPD has advantages over the other two phosphorus sensing GC detectors: 1) the Thermionic Specific Detector (TSD), a nitrogen-phosphorus detector, and 2) the Flame Photometric Detector (FPD). Comparisons are made between these detectors. Finally, high speed data acquisition firmware and software that allows one to easily set up the PFPD and review the pulsed emission data emanating from eac...

Migration patterns of nutrient elements, viz. (59)Fe, (54)Mn, (65)Zn, and (86)Rb, supplied to young corn (Zea mays) leaves were studied using a modified chromatogram scanner. It was found that the isotopes supplied to one-half of the leaf did not migrate to the other side across the midrib, but moved generally toward the base of the applied part of the leaf.

chromatography (UHPLC) has taken a firm foothold in the analytical laboratory. However, it is rare that 100% of instrumentation within a company is all from the same vendor and of the same model. Differences between equipment can lead to unexpected changes in the chromatogram when a method is transferred from one system to another. In this issue of Separation Science we examine these issues and...