Ethoxy (EO) chain nonylphenol dehydrogenase (NPEO-DH) from Ensifer sp. AS08 and EO chain octylphenol dehydrogenase from Pseudomonas putida share common molecular characteristics with polyethylene glycol (PEG) dehydrogenases (PEG-DH) and comprise a PEG-DH subgroup in the family of glucose-methanol-choline (GMC) oxidoreductases that includes glucose/alcohol oxidase and glucose/choline dehydrogena...

A signal amplification system for electrochemiluminescence (ECL) of quantum dots (QDs) was developed by using electrochemically reduced graphene oxide (ERGO) to construct a nanobiosensing platform. Due to the structural defects of GO, the ECL emission of QDs coated on GO modified electrode was significantly quenched. After the electrochemical reduction of GO, the restoration of structural conju...

The increased oxygen uptake of liver suspensions on the addition of acetylcholine, which was reported by Bernheim and Bernheim in 1933 (2), was shown by Mann and Quastel (3) to be due to the oxidation of choline. Betaine aldehyde was identified as the oxidation product (3). The choline oxidase (CO) system was found by Marm, Woodward, and Quastel (4) to consist partly of a typical dehydrogenase....

Glycine betaine (GB) is a compatible solute that is also capable of stabilizing the structure and function of macromolecules. Several GB-producing transgenic rice lines were generated in which the Arthrobacter pascens choline oxidase (COX) gene, fused to a chloroplast targeting sequence (TP) was expressed under the control of an ABA-inducible promoter (SIP; stress-inducible promoter) or a ubiqu...

A detector for the simultaneous determination of choline (Ch) and acetylcholine (ACh) based on a sensitive trienzyme chemiluminometric biosensor in a single line flow injection (FI) system is described. Immobilized choline oxidase (ChOx), immobilized peroxidase (POx), immobilized acetylcholinesterase, and coimmobilized ChOx/POx were packed, in turn, in a transparent ETFE tube (1 mm i.d., 75 cm)...

A preterm baby boy with blood and bone changes of copper deficiency is described. Copper deficiency was suspected after fracture of the left femur during examination of the hip joint. A low serum copper concentration (2.7 mumol/l; 17.2 micrograms/100 ml) and caeruloplasmin (0.04 g/l; 0.004 g/100 ml) confirmed the diagnosis. Despite the introduction of solids at 18 weeks the copper concentration...

Using cytochrome oxidase and esterase assays as a guide, mitochondria and microsomes were prepared from ox heart homogenate with about 25% cross-contamination of phospholipid. By the same criteria, the lipid complement of well washed myofibrils was essentially microsomal in origin. Approximately 60% of the phospholipid of the whole homogenate was found to be associated with microsomes, about ha...

The promising advantages of Prussian Blue (PB) as catalyst and of the thick film screen printing technology have been combined to assemble sensors with improved characteristics for the amperometric determination of H(2)O(2). PB-modified screen printed electrodes were applied to detect H(2)O(2) at an applied potential of -0.05 V versus the internal screen printed Ag pseudoreference electrode, sh...

two hundred and forty hubbard classic broiler chicks were used in a 35 day trial at chittagong veterinary and animal science university farm, bangladesh to study the effects of supplemental choline chloride on performance parameters, carcass characteristics and their association in commercial broiler. all birds had free access to ad libitum feeding. birds were fed four types of diet i.e. diet w...