infectious bursal disease virus (ibdv) is the causative agent of gumboro disease, an infectious disease of global economic importance in poultry. the expression of heterologous proteins in p.pastoris is fast, simple and inexpensive. in this study, vp2 encoding gene of classical d78 ibdv was amplified using reverse transcription (rt) polymerase chain reaction (pcr) and cloned into ppiczαa vector...

Infectious bursal disease virus (IBDV) is the causative agent of Gumboro disease, an infectious disease of global economic importance in poultry. The expression of heterologous proteins in P.pastoris is fast, simple and inexpensive. In this study, VP2 encoding gene of classical D78 IBDV was amplified using reverse transcription (RT) polymerase chain reaction (PCR) and cloned into pPICZαA vector...

the objective of this study was to compare the effect of two conventional h9n2 avian influenza (ai) vaccines on replication and shedding of the h9n2 ai virus in broiler chickens. these inactivated oil emulsion vaccines contain either a uae or an iranian h9n2 ai isolate. one hundred and fifty one-day-old commercial broiler chickens were randomly divided into six groups. the birds, except for the...

The large amounts of feathers produced by the poultry industry, that is considered as a waste was explored for possible uses in various industries, such as meals for animals, biofuels, biodegradable plastic materials, combating water pollution and more. That review mentions these uses, but concentrate on the utilization of feathers for the diagnosis of viral infections and for monitoring vaccin...

this experimental study was carried out to induce a co-infection of h9 aiv and ibv with inoculumsprepared from tracheal scrubbed epithelium tissues in natural co-infected birds to rule out the effect of fieldundesired environmental conditions and also other infectious causative agents. eighty 1-day-old broilerswere randomly divided into four equal groups. at 21 days of age, three test groups we...

infectious bronchitis (ib) and newcastle disease (nd) are highly contagious and the most economically important diseases of the poultry affecting respiratory tract and causing economic losses in poultry industry throughout the world. in the present study, the simultaneous detection and differentiation of causative agents of these diseases were investigated using duplex-rt-pcr. rna was extracted...

Simple assay systems for infectivity titrations of avian infectious bronchitis virus (IBV) in chicken embryo trachea organ cultures (OC) were developed using plastic multiplate wells with one tracheal ring per well; these assays appeared to be much more satisfactory than the conventional rolled-tube method. The medium, 0.05 M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid)-buffered ...

Infectious bronchitis (IB) is one of the most common highly infectious viral respiratory diseases poultry having wide geographical distribution. Yet, little known about infection in northwestern states Nigeria. In this study, a total 263 pooled cloacal and tracheal swab samples were collected from apparently healthy avian species (duck, dove, geese, guinea fowl, local chicken, ostrich, parrot, ...

Infectious salmon anaemia virus (ISAV) is an orthomyxo-like virus that causes serious disease in Atlantic salmon (Salmo salar). Like the orthomyxoviruses, ISAV has been shown to possess haemagglutinin (HA) activity. This study presents the cloning, expression and identification of the ISAV HA gene, which was isolated from a cDNA library by immunoscreening. The HA gene contained an ISAV-specific...