نتایج جستجو برای: chemoenzymatic reaction

تعداد نتایج: 413126  

Journal: :Current Protocols in Nucleic Acid Chemistry 2004

Journal: :Journal of the American Chemical Society 2019

Journal: :Bioscience, Biotechnology, and Biochemistry 1992

Journal: :Molecules 2018
Theodore Tyrikos-Ergas Vasileios Giannopoulos Ioulia Smonou

Rugulactone is a natural product isolated from the plant Cryptocarya rugulosa. It has shown very important biological activity as an inhibitor of the nuclear factor κB (NF-κB) activation pathway. A new chemoenzymatic approach towards the synthesis of rugulactone is presented here. The chirality, induced to the key intermediate by a stereoselective enzymatic reduction utilizing NADPH-dependent k...

2014
Xin Meng Wenlong Yao Jiansong Cheng Xu Zhang Lan Jin Hai Yu Xi Chen Fengshan Wang Hongzhi Cao

A novel chemoenzymatic approach for the synthesis of disialyl tetrasaccharide epitopes found as the terminal oligosaccharides of GD1α, GT1aα, and GQ1bα is described. It relies on chemical manipulation of enzymatically generated trisaccharides as conformationally constrained acceptors for regioselective enzymatic α2-6-sialylation. This strategy provides a new route for easy access to disialyl te...

Journal: :Chemical communications 2012
Pierre Stallforth Stefan Matthies Alexander Adibekian Dennis G Gillingham Donald Hilvert Peter H Seeberger

A chemoenzymatic synthesis of sialic acid from inexpensive N-acetyl-D-glucosamine is described. In a three-step Wittig-protection-ozonolysis strategy manno-configured aldehydes are obtained. Treatment with oxaloacetate in the presence of macrophomate synthase affords the signature α-keto-γ-hydroxy acid moiety with high diastereoselectivity.

Journal: :The Journal of organic chemistry 2017
Victor L Schultz Xing Zhang Kathryn Linkens Jenna Rimel Dixy E Green Paul L DeAngelis Robert J Linhardt

Unnatural uridine diphosphate (UDP)-sugar donors, UDP-4-deoxy-4-fluoro-N-acetylglucosamine (4FGlcNAc) and UDP-4-deoxy-4-fluoro-N-acetylgalactosamine (4FGalNAc), were prepared using both chemical and chemoenzymatic syntheses relying on N-acetylglucosamine-1-phosphate uridylyltransferase (GlmU). The resulting unnatural UDP-sugar donors were then tested as substrates in glycosaminoglycan synthesis...

Journal: :Nature chemical biology 2014
Yongmei Xu Chao Cai Kasemsiri Chandarajoti Po-Hung Hsieh Lingyun Li Truong Q Pham Erica M Sparkenbaugh Juzheng Sheng Nigel S Key Rafal Pawlinski Edward N Harris Robert J Linhardt Jian Liu

Low-molecular-weight heparins (LMWHs) are carbohydrate-based anticoagulants clinically used to treat thrombotic disorders, but impurities, structural heterogeneity or functional irreversibility can limit treatment options. We report a series of synthetic LMWHs prepared by cost-effective chemoenzymatic methods. The high activity of one defined synthetic LMWH against human factor Xa (FXa) was rev...

2014
Haixin Ding Wei Li Zhizhong Ruan Ruchun Yang Zhijie Mao Qiang Xiao Jun Wu

We report the first total synthesis of trachycladines A (10 steps, 34.2% overall yield) and B (11 steps, 35.0% overall yield) by using 5-deoxy-1,2,3-tri-O-acetyl-β-D-ribofuranose as the starting material. The critical step was the SnCl4 assisted regio- and steroselective deprotection of perbenzylated 1-O-methyl-5-deoxyribofuranose. The enzyme adenylate deaminase (EC 3.5.4.6) was successfully ap...

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