In order to know the influence of the surface characteristics and the chain properties on the adsorption of amphiphilic molecules onto polystyrene latex, a set of experiments to study the adsorption of ionic surfactants, nonionic surfactants and an amphiphilic synthetic peptide on different latex dispersions was performed. The adsorbed amount versus the equilibrium surfactant concentration was ...

During in vitro transcription of T4 DNA by E. coli RNA polymerase, chain initiation stops coincidentally with synthesis at low ionic strength (0.11) with an average of one RNA chain initiated per 24S polymerase molecule. At high ionic strength (0.37), initiation as well as synthesis continues for several hours, with an average of four chains initiated per enzyme molecule in two hours. The RNA p...

For study of time-dependent conformation, all previous single-molecule imaging studies of polymer transport involve fluorescence labeling uniformly along the chain, which suffers from limited resolution due to the diffraction limit. Here we demonstrate the concept of submolecular single-molecule imaging with DNA chains assembled from DNA fragments such that a chain is labeled at designated spot...