UNLABELLED The promising antineoplastic agent diaziquone is associated with prolonged aplasia and rare instances of bone marrow necrosis, but only mild extramedullary toxicity. To explore the drug's potential as a myeloablative agent prior to bone marrow transplantation, we compared its effects on hematopoietic versus marrow stromal cells. After short-term (one to six hours) or prolonged (three...

A series of experiments was designed determining the seeding and ultimate to study the effect of spleen size on retention of CFU in the spleen of a the seeding and retention of spleen recipient mouse is the size of the colony-forming units (CFU) after inspleen. It is concluded that the most jection into irradiated mice. The cellureasonable value of the fraction’ of larity of the spleens of reci...

Aims: To assesses the effectiveness of surgical handwashing (S-HW) in healthcare workers (HCWs) terms organic material and bacteria elimination by means bioluminescence assays.
 Methods: 62-HCWs were subjected to analyses determine adenosine triphosphate (ATP) colony forming units (CFU) Enterobacteriaceae (EB) E. coli before after S-HW. A checklist evaluate adherence hand hygiene (HH) prot...

The aorta-gonad-mesonephros (AGM) region is a potent hematopoietic site in the midgestation mouse conceptus and first contains colony-forming units-spleen day 11 (CFU-S(11)) at embryonic day 10 (E10). Because CFU-S(11) activity is present in the AGM region before the onset of hematopoietic stem cell (HSC) activity, CFU-S(11) activity in the complex developing vascular and urogenital regions of ...

The aorta-gonad-mesonephros (AGM) region is a potent hematopoietic site in the midgestation mouse conceptus and first contains colony-forming units–spleen day 11 (CFU-S11) at embryonic day 10 (E10). Because CFU-S11 activity is present in the AGM region before the onset of hematopoietic stem cell (HSC) activity, CFU-S11 activity in the complex developing vascular and urogenital regions of the AG...

Culture of a mixture of bone marrow cells with and without T6 chromosome marker in diffusion chambers in mice yielded colonies (CFU-DG) containing cells of a single karyotype, suggesting clonality. Injection of individual CFU-DG colonies into lethally irradiated mice resulted in increased spleen colony formation on day 12 (CFU-S). The possibility of endogenous origin was excluded by demonstrati...

The production of mature blood cells is finely regulated, so that the homeostasis can be maintained and cell production be adapted to the specific needs of particular blood cell compartments (e.g., granulocytes during infection). A complex regulatory network of positive and negative signals and cellular interactions serves the hematopoietic system to fulfill this role. In vitro colony assays ar...

Currently used industry testing programs require the ability to detect Escherichia coli O157:H7 in samples of beef trim or ground beef at levels as low as 1 CFU/375 g. We present a reliable protocol for generating a control inoculum for verification testing at this low concentration and evaluate its use. Results show that half of all samples received no cells when 1 CFU was the target concentra...

The effects of two different potent inhibitors of protein kinase C, 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7) and staurosporine on human myeloid (CFU-C) and late erythroid progenitor cells (CFU-E) were studied using an in vitro clonal assay. Our objective was to determine whether protein kinase C has a role in signal transduction related to proliferation of these committed progenitor...

Murine erythroid colony-forming units (CFU-E) representing successive cell generations in a six-generation long in vitro maturation sequence were tested for their response to erythropoietin (Epo) by measurement of Epo-exposure times necessary to stimulate heme biosynthesis. Generation I CFU-E, which produce mainly 32-cell erythroid colonies, were isolated in 82% average purity from spleens of t...