1. A high speed centrifuge is described in which the speed is limited only by the strength of the material of which the rotor is made. It carries sixteen tubes, each of which conveniently accommodates 7 cc. of fluid. 2. The centrifuge operates in a very high vacuum and therefore requires only a small amount of driving energy. The arrangement has been found to eliminate the possibility of produc...

In principle, equilibrium analytical ultracentrifugation (AU) can be used to quantify the binding stoichiometry and affinity between small-molecule ligands and proteins in aqueous solution. We show here that heteromeric binding constants can be determined using a data-fitting procedure which utilizes a postfitting computation of the total amount of each component in the centrifuge cell. The met...

A buried pipeline can buckle upwards if there is not enough resistance to movement. Both the uplift resistance (the maximum force per unit length) and the mobilisaion distance (the distance the pipeline has move before the maximum force is reached) are important in design. Tests on pipes in several soils were carried out at 1 g at full scale and in a centrifuge at 10 g and 1/10 scale. A compari...

Vulnerability to cavitation is a key variable defining the limits to drought resistance in woody plants (Kursar et al., 2009). This trait is typically assessed by a vulnerability curve, which can be generated by a range of methods, including dehydration (Sperry et al., 1988), air injection (Cochard et al., 1992), and centrifugation (Alder et al., 1997). Results from two recent papers suggest th...

classical soil mechanics involves the study of fully saturated soils. however, many problems encountered in geotechnical engineering practice involve unsaturated soil, in which behavior is significantly different from classical saturated soil. negative pore pressure and capillary forces develop a virtual cohesion between the grains of semi saturated soils. this kind of cohesion is dependent on ...

Several methods have been evaluated for the separation of red cells of different ages on the basis of density differences. Simple sedimentation and flotation technics have been found to be unsatisfactory. Centrifugation, followed by a modified sampling procedure, has been demonstrated to provide excellent separation of erythrocyte populations of different ages. Standardization of centrifuge con...

Sucrose density-gradient analysis is one method of identifying specific estrogen and progesterone receptors in mammary tumor tissue. Use of the vertical rotor makes this practical for routine applications in the clinical laboratory by increasing the number of samples that can be run at one time and shortening the centrifuge time from 18 to less than 2 h. The separations and reproducibility comp...

objective: pseudomonas aeruginosa is the major cause of septicemia and wound infection in burned patients. immunotraphy is the best practical way for prevention and treatment of these infections. flagella as one of the most important bacterial virulence factors has important role in attachment, motility, chemotaxis and tlr-5-dependent immune response so that it propounded as a vaccine candidate...