The final steps in butyrate synthesis by anaerobic bacteria can occur via butyrate kinase and phosphotransbutyrylase or via butyryl-coenzyme A (CoA):acetate CoA-transferase. Degenerate PCR and enzymatic assays were used to assess the presence of butyrate kinase among 38 anaerobic butyrate-producing bacterial isolates from human feces that represent three different clostridial clusters (IV, XIVa...

Cellulose and cellulose acetate/Fe2TiO5 nanocomposites have been synthesized successfully. Nanocomposites indicate a ferromagnetic paramagneticbehavior, as evidenced by using vibrating sample magnetometer (VSM) atroom temperature. Fourier transform infrared spectrometry (FTIR), scanning electron microscopy (SEM) and simultaneous thermal analysis (STA) respectively, to characterize,diagnosis, mo...

This work evaluated the effects of condensable syngas impurities on cell viability and product distribution Butyribacterium methylotrophicum in fermentation. The condensates were collected during gasification two technical lignins derived from wheat straw (WST) softwood (SW) at different temperatures presence or absence catalysts. cleanest with 169 3020 ppmv H2S NH3, respectively, was obtained ...

An endospore-forming, butyrate-degrading bacterium (strain BH) was grown on butyrate in monoxenic coculture with a methanogen. The culture formed dense aggregates when Methanobacterium formicicum was the methanogenic partner, but the culture was turbid when Methanospirillum hungatei was the partner. In contrast, a propionate-degrading, lemon-shaped bacterium (strain PT) did not form aggregates ...

the objective of this meta-analysis was to compare effects of chemical methanogenic inhibitors on fermentation parameters in ruminal batch cultures. nine published in vitro studies including 26 treatment means in which 2-bromoethane sulfonate, bromochloromethane and 9,10-anthraquinone were used as methanogens inhibitor compared in amount of ch4, h2, total volatile fatty acid, actate, propionate...

In order to elucidate the role of propionate in themetabolism of acetate and butyrate in rumen epithelium, the effects of propionate addition on the "C transfer from "C-acetate and -butyrate into CO2, glucose, ketone bodies and lipids fractions in rumen epithelium of normally fed, starved and alloxan diabetic sheepwere investigated. The experimental results are summarized as follows.1) In rumen...

Abstract The intestinal microbiota contributes to gut immune homeostasis, where short-chain fatty acids (SCFAs) function as the major mediators. We aimed elucidate immunomodulatory effects of acetate, propionate, and butyrate. With that in mind, we sought characterise expression SCFA receptors transporters well SCFAs’ impact on activation different cells. Whereas all three SCFAs decreased tumou...

We demonstrated the feasibility of fedbatch operation using Clostridium acetobutylicum suspension culture as a biocatalyst for the continuous production of hydrogen. The optimum operating pH and temperature of the current cultivation system for hydrogen production were pH 6.0 and 37 degrees C, respectively. The volumetric loading of the bioreactor for hydrogen production can be as high as 650 m...

We investigated the distribution of short chain fatty acids (SCFA) in enema samples taken from subjects before sigmoidoscopy as an indicator of possible microbial community differences between subjects subsequently diagnosed as normal or having colonic disorders. The major SCFA in all groups were acetic, propionic, and butyric acids. A significantly higher ratio of acetate to total SCFA and low...