Cell separation and sorting are essential steps in cell biology research and in many diagnostic and therapeutic methods. Recently, there has been interest in methods which avoid the use of biochemical labels; numerous intrinsic biomarkers have been explored to identify cells including size, electrical polarizability, and hydrodynamic properties. This review highlights microfluidic techniques us...

Correction for 'Continuous inertial microparticle and blood cell separation in straight channels with local microstructures' by Zhenlong Wu et al., Lab Chip, 2016, 16, 532-542.

We report the first digital microfluidic (DMF) system capable of impedance sensing of mammalian cells. The new system was validated in three assays: calibration, proliferation, and serum sensing. In the first assay, three cell lines (HeLa, CHO-K1, and NIH-3T3) were seeded at different densities to determine the relationship between impedance and cell number, which was found to be linear for eac...

background and objectives : the attempts were made to describe the development of a whole cell immobilization of p. pastoris by entrapping the cells in polyacrylamide gel beads. the alcohol oxidase activity of the whole cell pichia pastoris was evaluated in comparison with yeast biomass production. materials and methods : methylotrophic yeast p. pastoris was obtained from collection of standard...

BACKGROUND Several processes of plant development, such as abscission, pollen release, fruit dehiscence, and seed dispersal, require organs or tissues to physically disassociate or split open. Due to the immobility of plant cells, these processes occur through coordinated mechanisms of cell separation that are not found in animals. Arabidopsis produces dry dehiscent fruits (siliques) making it ...

Single-cell phenotyping is critical to the success of biological reductionism. Raman-activated cell sorting (RACS) has shown promise in resolving the dynamics of living cells at the individual level and to uncover population heterogeneities in comparison to established approaches such as fluorescence-activated cell sorting (FACS). Given that the number of single-cells would be massive in any ex...