objective: to study the mechanisms involved in amyloid formation processes, we made a mammalian cell culture model of amylin aggregation and characterized its properties. materials and methods: amyloid fibrils were extracted from cho cells and binding affinity to thioflavin t and congo red was investigated. then the apple-green birefringence of extracted fibrils was detected under polarized li...

Background: Rad51 is a protein with critical role in double strand break repair. Down-regulation of this protein has a significant effect in radiosensitivity of some cell lines like prostate carcinoma. Compared to monolayer cell culture model, the spheroids are more resistant to radiation. The aim of the current study was to determine the Rad51 protein level in DU145 spheroids, and monol...

with the growth in customers’ demand diversification, mixed-model u-lines (mmul) have acquired increasing importance in the area of assembly systems. there are generally two different approaches in the literature for balancing such systems. some researchers believe that since the types of models can be very diverse, a balancing approach without simultaneously sequencing of models will not yield...

the aim of this study was to evaluate and compare the cytotoxicity induced by two resin-based sealers, 2seal and ah plus, in two osteoblast-like cell lines, mg-63 and saos-2. using sterile discs of both sealers in complete media, 24- and 72-h extracts were prepared. the extracts were exchanged with saos-2 or mg-63 cell culture media at 75% confluence, and after 24 h incubation, cell viability t...

in spite of serious cardiotoxicity side-effects, doxorubicin is frequently used for treatment of several types of cancers. isolated human adult cardiomyocytes could be the best model for assessing drug-induced cardiotoxicity, while harvesting mature cardiomyocytes is restricted by some limitations such as biopsy size, cell numbers, viability, proliferative capacity and their disability to be pa...

objective: bone marrow mesenchymal stem cells reside in bone marrow and support homing and differentiation of hematopoietic stem cells (1). this makes them an excellent instrument for regenerative treatment and co-culture with the hsc. materials and methods: in this study, k562 cell lines were treated with butyric acid(for erythroid differentiation), co-cultured with mesenchymal stem cells and ...