BACKGROUND The detection of unknown mutations is important in research and medicine. For this purpose, a mismatch-specific endonuclease CEL I from celery has been established as a useful tool in high throughput projects. Previously, CEL I-like activities were described only in a variety of plants and could not be expressed in an active form in bacteria. RESULTS We describe expression of activ...

Chronic eosinophilic leukaemia (CEL) is a rare myeloproliferative disorder of unknown etiology characterized by an autonomous, clonal proliferation of eosinophilic precursors resulting in persistently increased number of eosinophils in the peripheral blood and bone marrow. There is vaguely overlapping clinico – pathological picture of CEL with idiopathic hypereosinophilic syndrome (HES) which o...

Purified non-starch polysaccharides (NSP) affect intestinal nutrient flow and hence microbial fermentation in pigs. Therefore, we investigated the effect of NSP differing in viscosity and fermentability on colonic fermentation and bacterial populations in eight ileal-cannulated barrows (BW 30 kg) fed a cornstarch-casein based diet supplemented with 5% low fermentable , low viscous cel lulose (C...

....................................................................... 10 1. REVIEW OF THE LITERATURE ........................................ 11 1.1 RET RECEPTOR TYROSINE KINASE.................................. 11 1.1.1 RET gene and RET proteins............................................. 11 1.1.2 RET is a functional receptor for GDNF-family ligands (GFLs).... 12 1.1.3 RET-dependent GFLs si...

BACKGROUND The aim of the present study was to investigate the role of microRNA (miRNA) let-7a in down-regulation of β1-adrenoceptors (β1-AR) and elucidate the underlying mechanism of chronic ischemia heart failure (CIHF) in rats. METHODS AND RESULTS CIHF model was established by occlusion of coronary artery for 4 weeks. β1-AR level was obviously down-regulated and let-7a up-regulated in the ...

Let-7 is one of the earliest discovered microRNAs (miRNAs) and has been reported to regulate self renewal and tumorigenicity of breast cancer cells. Let-7a is a member of this family and its function has not been fully characterized in breast cancer. First, total RNAs of breast cancer cells (MDA-MB-231, MCF-7), breast cancer tissues and corresponding adjacent normal tissues were extracted and u...

MicroRNA-7a/b (miR-7a/b) protects cardiac myocytes from apoptosis during ischemia/reperfusion injury; however, its role in angiotensin II (ANG II)-stimulated cardiac fibroblasts (CFs) remains unknown. Therefore, the present study investigated the anti-fibrotic mechanism of miR-7a/b in ANG II-treated CFs. ANG II stimulated the expression of specific protein 1 (Sp1) and collagen I in a dose- and ...

The relationship between the pri-let-7a-1 rs10739971 polymorphism and gastric cancer (GC) risk has been reported. However, the role of this polymorphism in the prognosis of GC remains largely elusive. Sequenom MassARRAY platform method and the polymerase chain reaction (PCR)-restriction fragment length polymorphism were used to investigate pri-let-7a-1 rs10739971 G→A in 334 GC patients. Real-ti...

Abnormal expression of let-7a microRNA (miRNA) in non-small cell lung cancer (NSCLC) cells and tissue has been previously reported. Our objective was to investigate whether let-7a miRNA is aberrantly expressed in the blood of NSCLC patients. Using real-time PCR (RT-PCR), we analyzed let-7a miRNA in archived whole blood from 65 participants, 35 of whom had NSCLC and 30 of whom did not. Using RT-...