Protein conformational heterogeneity and dynamics are known to play an important role in enzyme catalysis, but their influence has been difficult to observe directly. We have studied the effects of heterogeneity in the catalytic reaction of pig heart lactate dehydrogenase using isotope edited infrared spectroscopy, laser-induced temperature jump relaxation, and kinetic modeling. The isotope edi...

Steady-state isotopic-transient kinetic analysis (SSITKA) is a very useful technique for the kinetic study of heterogeneous catalytic reactions. Initially developed by Happe1,l Bennett,2 and Biloen? SSITKA has been used in a number of studies to determine in situ kinetic information about the reaction mechanism and the catalyst-surface reaction intermediates. The technique is based upon the det...

The net rate of metabolic processes in cells depends on the competition between the reactivity of the system and the diffusive flow of substrates to the reaction center (Weisz, 1973). For instance, aerobic metabolism depends on the kinetic properties of the mitochondrial enzymes involved in oxidative phosphorylation and on the diffusive flux of substrates such as ADP to the mitochondria. Howeve...

cis-polybutadiene was functionalized using in–situ generated dmd as oxidant and tetra-n-butyl ammonium bromide as phase transfer catalyst (ptc). the epoxidation process was investigated by 1hnmr spectroscopy. 1hnmr spectrum analysis of products confirms the absence of ring opening side products in epoxidation reaction. various factors such as reaction temperature, reaction time and the concentr...

The mechanism of action of tentoxin on the soluble part (chloroplast F1 H+-ATPase; CF1) of chloroplast ATP synthase was analyzed in the light of new kinetic and equilibrium experiments. Investigations were done regarding the functional state of the enzyme (activation, bound nucleotide, catalytic turnover). Dialysis and binding data, obtained with 14C-tentoxin, fully confirmed the existence of t...

The review contains a comparative analysis of studies on the production hydrogen and syngas based processes partial oxidation natural gas other types feedstock. results presented in literature show high potential non-catalytic autothermal hydrocarbons for development chemistry energetics. makes it possible to overcome such serious shortcomings traditional technologies as technological complexit...

Water molecules can enhance or inhibit hydrogenation reactions depending on the nature of reactive species and active sites. In metal-catalyzed nitrite (NO2–) reduction presence protons is essential to complete reaction in aqueous phase. By coupling rigorous kinetics studies Pd with kinetic isotope theoretical calculations we have shown that, contrary previously proposed mechanisms surface H-in...