We describe a solid-phase enzyme immunoassay for ferritin in human serum, with use of a horseradish peroxidase-labeled antibody and a highly sensitive chromogen, 2,2'-azino-di(3-ethyl-benzthiazoline-6-sulfonate). The assay requires only 10 microL of serum per assay, relatively less time and labor than other assys for ferritin, and as little as 10 pg of ferritin can be measured. We believe this ...

A simple spectrophotometric method is reported her for the estimation of reserpine in polyherbal formulations. Estimation is based on the reaction with3-methylbenzolinone-2hydrazone (MBTH) reagent in presence of cerric ammonium sulphate to yield a violet coloured chromogen, which exhibits and absorption maxima at 580 nm. The chromogen is stable for 10 minutes.

Mike Levine is at the University of California, Berkeley where he is coDirector of the newly formed Center for Integrative Genomics. He obtained his Ph.D. from Yale University with Alan Garen studying steroid-regulated gene expression in Drosophila larvae. As a Jane Coffin Childs fellow he did postdoctoral research with Walter Gehring at the University of Basel in 1982. After a second short pos...

A rapid and sensitive spectrophotometric method is proposed for determination of metronidazole and secnidazole. The method depends on the reduction of metronidazole and secnidazole molecule with zinc dust and hydrochloric acid flowed by diazotization and coupling with 8-quinolinol to give red colored chromogens easily measured spectrophotometrically which has lambda(max) = 500 nm. The experimen...

New, simple and sensitive spectrophotometric methods for the determination of rosiglitazone and pioglitazone have been developed. The method is based on the oxidation of 2, 4-dinitrophenyl hydrazine (2, 4DNP) and coupling of the oxidized product with rosiglitazone and pioglitazone to give intensely colored chromogen. Rosiglitazone and pioglitazone showed maximum absorbance at 421 nm and 480 nm ...

The aim of study was to develop and validate two simple, sensitive, and extraction-free spectrophotometric methods for the estimation of risperidone in both pure and pharmaceutical preparations. They are based on the charge transfer complexation reactions between risperidone (RSP) as n-electron donor and p-chloranilic acid (p-CA) in method A and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) i...

Background: Recently, concerns have been raised regarding the environmental and public health safety of azo dyes, most widely used synthetic dyes. The membrane technique has introduced as one efficient methods for dye removal treatments. Polyvinylidene fluoride (PVDF) manipulated by surfactants was studied dye, carmoisine. Methods: PVDF prepared via non-solvent-induced phase separation (NIPS) t...

The interaction of native calf thymus DNA (CTDNA) with azorubine in 10 mM Tris–HCl aqueous solution at neutral pH 7.4 was monitored by UV-vis absorption and voltammetric techniques. Upon addition of the azorubine, UV absorption band of DNA decreased. After addition different concentrations of CT-DNA, UV absorption band of azorubine showed hyperchromisity. The binding constants (Kb) for the azor...

Three simple, accurate and economical spectrophotometric methods in ultraviolet and visible region were developed for the determination of cefprozil in bulk and dosage forms have been described. The method A is based on the reduction of ferric ion into ferrous ion by the mentioned drug in the presence of 1,10-phenanthroline to form a highly stable orange red colored ferrion complex measured at ...