We have examined the induction of carbonic anhydrase activity in Chlamydomonas reinhardtii and have identified the polypeptide responsible for this activity. This polypeptide was not synthesized when the alga was grown photoautotrophically on 5% CO(2), but its synthesis was induced under low concentrations of CO(2) (air levels of CO(2)). In CW-15, a mutant of C. reinhardtii which lacks a cell w...

Diabetes-associated complications in the microvasculature of the brain are caused by oxidative stress, generated by overproduction of reactive oxygen species from hyperglycemia-induced accelerated oxidative metabolism of glucose. Pericytes, essential for the viability of the microvasculature, are especially susceptible to oxidative stress. Mitochondrial carbonic anhydrases, regulators of the ox...

In recent years there has been renewed activity in the literature concerning the 1,3-dipolar cycloaddition reaction (1,3-DCR) of organic azides (R-N₃) with alkynes (R'-C≡CH) to form 1,2,3-triazoles, i.e. the Huisgen synthesis. The use of catalytic Cu(I) leads to a dramatic rate enhancement (up to 10(7)-fold) and exclusive synthesis of the 1,4-disubstituted 1,2,3-triazole product. The reaction, ...

experimental acute gastric ulcer is produced by the intraperitoneal injection of a single dose of indomethacin. acetazoleamide, a potent carbonic anhydrase inhibitor, has significantly prevented the occurrence of these ulcers. its probable mechanism is discussed.

The cover shows an artistic concept describing evolutional approach to the generation of dynamic inhibitors and activators Carbonic Anhydrase on both molecular supramolecular levels through implementation reversible covalent reactions non-covalent intermolecular interactions within inner active catalytic center outer enzyme microenvironment, respectively. They open up wide perspectives construc...

10.2217/14750708.4.3.355 © 2 Inhibitors of the zinc enzyme carbonic anhydrase (CA) targeting different isozymes among the 15 presently reported in humans have various therapeutic applications. These enzymes are efficient catalysts for the hydration of carbon dioxide to bicarbonate and protons, playing crucial physiological/pathological roles related to acid–base homeostasis, secretion of electr...

BACKGROUND Carbonic anhydrase IX is a member of α-carbonic anhydrases that is preferentially expressed in solid tumors. It enables bicarbonate transport across the plasma membrane, neutralizing intracellular pH and conferring to cancer cells a survival advantage in hypoxic/acidic microenvironments. Overexpression of carbonic anhydrase IX in cancer tissues is regulated by hypoxia inducible facto...