The witches’ broom disease of lime trees is a destructing disease caused by the bacterium Candidatus <span style="font-variant: normal; fon...

During 2021 surveys, greengage witches’-broom (GWB) and decline (GD) diseases were respectively observed in Fars Yazd provinces of Iran. Presence phytoplasma the symptomatic plants was confirmed by PCR amplifications 16S rRNA gene. Sequence analyses showed affiliation GWB GD phytoplasmas to‘Candidatus Phytoplasma phoenicium’ (16SrIX-B) and‘Ca. P. aurantifolia’ (16SrII-C) related strains, respec...

Phytoplasma phylogenetics has focused primarily on sequences of the non-coding 16S rRNA gene and the 16S-23S rRNA intergenic spacer region (16-23S ISR), and primers that enable amplification of these regions from all phytoplasmas by PCR are well established. In this study, primers based on the secA gene have been developed into a semi-nested PCR assay that results in a sequence of the expected ...

Phytoplasmas are bacterial phytopathogens that release virulence effectors into sieve cells and act systemically to affect the physiological and morphological state of host plants to promote successful pathogenesis. We show here that transgenic Nicotiana benthamiana lines expressing the secreted effector SAP11 from Candidatus Phytoplasma mali exhibit an altered aroma phenotype. This phenomenon ...

In Europe the most devastating phytoplasma associated with grapevine yellows (GY) diseases is a quarantine pest, flavescence dorée (FDp), from the 16SrV taxonomic group. The on-site detection of FDp with an affordable device would contribute to faster and more efficient decisions on the control measures for FDp. Therefore, a real-time isothermal LAMP assay for detection of FDp was validated acc...

Tancik J., Seljak G. (2017): Occurrence of Scaphoideus titanus Ball and some other Auchenorrhyncha in the vineyards of western Slovakia. Plant Protect. Sci., 53: 95–100. A study of Auchenorrhyncha was carried out in 2014 and 2015 in 7 vineyard plots with different varieties and pest management strategies in the Nitra wine region and Lesser Carpathian wine region in western Slovakia. The aim of ...

The leafhopper Scaphoideus titanus is the vector of 'Candidatus Phytoplasma vitis', the causal agent of Flavescence dorée (FD) a key disease for European viticulture. In organic vineyards, the control of S. titanus relies mostly on the use of pyrethrins that have suboptimal efficacy. During 2016, three field trials were conducted to evaluate the efficacy of kaolin, orange oil, insecticidal soap...

A working group established in the framework of the EUPHRESCO European collaborative project aimed to compare and validate diagnostic protocols for the detection of "Flavescence dorée" (FD) phytoplasma in grapevines. Seven molecular protocols were compared in an interlaboratory test performance study where each laboratory had to analyze the same panel of samples consisting of DNA extracts prepa...

During the autumn seasons of 2016-2019, several surveys were carried out in grapevine (Vitis vinifera L.) cultivation areas ?anl?urfa and Ad?yaman provinces South-eastern part Turkey, to identify plants exhibiting phytoplasma-like symptoms including yellowing, small leaf formation, chlorosis, short internodes, severe redness inward curling detect characterize causal agent responsible for these ...