In this study, third-harmonic generation (THG) imaging measurements were performed to characterize different developmental stages of the nematode Caenorhabditis elegans (C. elegans) embryos. Femtosecond laser pulses (1028 nm) were utilized for excitation. THG image contrast modality proved as a powerful diagnostic tool, providing valuable information and offering new insights into the complex d...

It is often assumed that the mutation rate is an evolutionarily optimized property of a taxon. The relevant mutation rate is for mutations that affect fitness, U, but the strength of selection on the mutation rate depends on the average effect of a mutation. Determination of U is complicated by the possibility that mutational effects depend on the particular environmental context in which the o...

Using the nematode Caenorhabditis elegans as a model organism, we have prepared cell-free extracts which accurately transcribe cloned homologous 5S RNA genes in vitro. These extracts also transcribe cloned tRNA genes, and actively process the resulting products. Unlike tRNA genes, transcription of 5S DNA shows some species specificity: C. elegans extracts do not transcribe Xenopus 5S RNA genes,...

The soil nematode Caenorhabditis briggsae is an attractive model system for studying evolution of both animal development and behavior. Being a close relative of C. elegans, C. briggsae is frequently used in comparative studies to infer species-specific function of the orthologous genes and also for studying the dynamics of chromosome evolution. The genome sequence of C. briggsae is valuable in...

We constructed homology models from the crystal structure of bovine Arp2/3 complex and sequences from six phylogenetically diverse species (Arabidopsis thaliana, Caenorhabditis elegans, Dictyostelium discoideum, Drosophila melanogaster, Saccharomyces cerevisiae, Schizosaccharomyces pombe) representing over 800 million years of evolution and used conserved surface residues to search for function...

Mutations that increase the longevity of the soil nematode Caenorhabditis elegans could define genes involved in a process specific for aging. Alternatively, these mutations could reduce animal metabolic rate and increase longevity as a consequence. In ectotherms, longevity is often negatively correlated with metabolic rate. Consistent with these observations, environmental conditions that redu...

Two sets of LSGs were identified using BLAST: Caenorhabditis elegans species-specific genes (SSGs, 1423), and Caenorhabditis genus-specific genes (GSGs, 4539). The data contained in this article show SSGs and GSGs have significant differences in evolution and that most of them were formed by gene duplication and integration of transposable elements (TEs). Subsequent observation of temporal expr...

Introduction, detection et isolement de mutants thermosensibles létaux etlou stériles chez les nématodes. I . Le némafode libre Caenorhabditis elegans. Grâce à l'élaboration d'un protocole original de détection et d'isolement des mutations thermosensibles létales etJou stériles, spécifique du nématode hermaphrodite autofécond Caenorhabditis elegans, souche Bergerac (Abdulkader & Brun, 1976), 25...

Citation: Du X, Huang Q, Guan Y, Lv M, He X, Fang C, Wang X and Sheng J (2018) Caffeine Promotes Conversion of Palmitic Acid to Palmitoleic Acid by Inducing Expression of fat-5 in Caenorhabditis elegans and scd1 in Mice. Front. Pharmacol. 9:321. doi: 10.3389/fphar.2018.00321 Caffeine Promotes Conversion of Palmitic Acid to Palmitoleic Acid by Inducing Expression of fat-5 in Caenorhabditis elega...

We propose to investigate the effect of electromagnetic fields on the moss Physcomitrella patens, and on the nematode Caenorhabditis elegans in the range of 900 MHz – 1 GHz. Physcomitrella patens is a plant whose developmental biology and genetics is well known and the nematode Caenorhabditis elegans has been completely sequenced and this organism has been and is still the most well known chord...