Ceramides can be delivered to cultured cells without solvents in the form of complexes with cholesteryl phosphocholine. We have analysed the delivery of three different radiolabeled D-erythro-ceramides (C6-Cer, C10-Cer and C16-Cer) to HeLa cells, and followed their metabolism as well as the cell viability. We found that all three ceramides were successfully taken up by HeLa cells when complexed...

Human alpha defensins are a class of antimicrobial peptides with additional antiviral activity. Such antimicrobial peptides constitute a major part of mammalian innate immunity. Alpha defensins contain six cysteines, which form three well defined disulfide bridges under oxidizing conditions. Residues C3-C31, C5-C20, and C10-C30 form disulfide pairs in the native structure of the peptide. The ma...

Neoplastic mouse lung epithelial cells contain greatly diminished activity, protein, and mRNA for the type I isozyme of cyclic AMP-dependent protein kinase (PKA I), while expression of the type II isozyme (PKA II) is similar to that of normal lung cells. A time course of PKA mRNA content in transcriptionally inhibited cells indicated that most PKA mRNAs are more stable in the neoplastic E9 cell...

This paper describes the separation and detection of five inorganic anions (I - , BrO3 NO2 Br NO3 -) by capillary ion chromatography using 1-alkyl-3-methylimidazolium-coated columns UV at 210 nm. The salts imidazolium (IM) ions (C2-, C4-, C6-, C8-, C10-, C18-IMs) are a family ionic liquids were newly examined as permanent coating reagents on triacontyl (C30) -bonded silica reversed phase column...

The effect of supplementing piglet diets with acidifiers containing the short-chain fatty acids - SCFA (propionic C3 and formic) together with medium-chain fatty acids -- MCFA (caprylic C8 and capric C10) on performance, nutrient apparent digestibility, intestinal microflora and small intestine structure was investigated. The study was performed on 326 piglets allocated to 5 experimental groups...

Nitrobacter winogradskyi is a chemolithotrophic bacterium that plays a role in the nitrogen cycle by oxidizing nitrite to nitrate. Here, we demonstrate a functional N-acyl-homoserine lactone (acyl-HSL) synthase in this bacterium. The N. winogradskyi genome contains genes encoding a putative acyl-HSL autoinducer synthase (nwi0626, nwiI) and a putative acyl-HSL autoinducer receptor (nwi0627, nwiR...

A liquid chromatography-mass spectrometry (LC/MS) method was developed for the separation and determination of linear alkylbenzenesulfonates (C10-C14 LAS) in environmental water samples using a hydrophilic polymer column (Shodex Mspak GF-310 4D). This method involves a solid-phase extraction of the LAS samples with a Sep-Pak PS-2 cartridge. The LAS components were separated on the column with a...

Advanced melanoma is the most aggressive form of skin cancer. It is highly metastatic and dysfunctional in melanogenesis; two processes that are induced by H2O2. This work presents a melanoma cell model with low levels of H2O2 induced by catalase overexpression to study differentiation/dedifferentiation processes. Three clones (A7, C10 and G10) of human A375 amelanotic melanoma cells with quite...

We evaluated an acinar cell line (SMG-C10) cloned from rat submandibular glands as a possible model for 1-adrenoceptor regulation of submandibular function. 1-Adrenoceptors are subdivided into three subtypes called 1A, 1B, and 1D, which can be distinguished from one another by their differential affinity values for subtype-selective 1-adrenoceptor antagonists. Thus, 1-adrenoceptor subtypes in S...