Escherichia coli B strains that have acquired the malB region from E. coli K-12 are able to utilize maltose and to adsorb phage lambda when grown at 30 C, but when grown at 40 C they do not absorb phage lambda and are devoid of amylomaltase activity. These Mal(ts) Lam(ts) cells can be mutated or transduced to become able to grow on maltose at 40 C, but they still have no detectable amylomaltase...

Cu-doped amorphous calcium phosphate (ACP) coatings were fabricated on the surface of pure titanium (Ti) by electrochemical deposition at initial electrolyte temperatures 35, 45, and 55°C. The antibacterial activities then evaluated plate counting method using Escherichia coli as indicator. Cu concentrations surfaces samples are increased from 6.90 to 15.05 mass% temperature is 35 ACP show that...

We examined the biotransformation of enantiomeric pairs of enones such as pulegone and carvone in recombinant Escherichia coli expressing Nicotiana tabacum pulegone reductase. It was found that recombinant E. coli cells acquired the ability for stereospecific hydrogenation of the exocyclic C=C double bond of pulegone. However, stereospecificity in hydrogenation with the recombinant E. coli cell...

The discovery of RNA interference (RNAi) in C. elegans has had a major impact on scientific research, led to the rapid development of RNAi tools and has inspired RNA-based therapeutics. Astonishingly, nematodes, planaria and many insects take up double-stranded RNA (dsRNA) from their environment to elicit RNAi; the biological function of this mechanism is unclear. Recently, the E. coli OxyS non...

Campylobacter is now the most frequently reported cause of gastrointestinal disease in England and Wales, yet few isolates are characterized beyond the genus level. The majority of isolates are Campylobacter jejuni (90%), with most of the remainder being Campylobacter coli. We describe an adaptation of the Penner serotyping scheme in which passive hemagglutination has been replaced by detection...

The aim of this study was to determine the effect of temperature on the survival of Salmonella and E. coli in fermented liquid pig feed (FLF). Liquid feed, fermented with Lactobacillus plantarum, was challenged with Salmonella or E. coli (six serovars of each) at 20, 30 or 37 C. Temperature significantly affected the survival of Salmonella and E. coli. In FLF containing ca 230 mmol L lactic aci...

Lactic acid bacteria (LAB) exert antagonistic activities against diverse microorganisms, including pathogens. In this work, we aimed to investigate the ability of LAB strains isolated from food produce biofilms and inhibit growth surface colonization Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 at 10°C. The 100 EHEC NCTC12900 was evaluated in agar diffusion assays. Thirty-seven showed str...

A survey of Candida albicans indicated that the organism was present at all sites sampled in a rain forest stream and in near-shore coastal waters of Puerto Rico. In the rain forest watershed no relationship existed between densities of fecal coliforms and densities of C. albicans. At two pristine sites in the rain forest watershed both C. albicans and Escherichia coli survived in diffusion cha...

BACKGROUND Recently the Type VI secretion system (T6SS), which can play a significant role in bacterial survival and pathogenesis, was reported in Campylobacter spp., having the hcp gene as a key component. METHODS Campylobacteriosis is associated with the consumption of infected chicken meat. Our study aimed to explore the presence of T6SS in C. jejuni (n = 59) and C. coli (n = 57) isolates,...

New carbapenern antibiotics, C-19393 S2 and H2, have been found to be potent and broadspectrum inhibitors of /3-lactamases. Among 11 types of /3-lactamases tested, those from Escherichia coli (plasmid-bearing), Klebsiella pneumoniae, Proteus vulgaris, Serratia marcescens and Bacteroides fragilis were especially sensitive. They also inhibited cephalosporinases insensitive to clavulanic acid. The...