bilateral pulmonary nodules. Histological examination of a biopsy sample of the thigh mass suggested synovial sarcoma. Because of the metastatic pulmonary lesions 150 mg doxorubicin was given by intravenous injection. Three days later the haemoglobin concentration fell suddenly to 10-6 g/dl (reticulocytes 4-1%'). Results of Coombs tests were negative. Supravital staining (cresyl blue) of a bloo...

The covalent immobilization of synthetic or natural macromolecular compounds containing amino groups onto polystyrene (PS) solid surfaces is of great interest in diagnostic applications. A sensitive assay allowing the determination of reactive end groups is therefore a powerful tool for predicting the performance of the active surface. Recently, we reported the use of the Coomassie brilliant bl...

Previous studies have confirmed that oxidized adenosine triphosphate, a P2X7 receptor antagonist, attenuates lipopolysaccharide-mediated microglial activation and inflammatory expression following neuronal damage in rat brain. NaCl and temperature may affect the potency of oxidized adenosine triphosphate. Brilliant blue G is a derivative of a widely used food additive and has little toxicity. T...

The aim of the study is to analyze effect succinate-containing drugs on cellular composition blood in rats at rest, during free swimming and weight-loaded forced test.
 Materials Methods was carried out practically healthy sexually mature male Wistar (250–300 g). Animals received a drug (meso-2,3-dimercaptosuccinic acid) before (4 % body weight), after 12 hours exercise. Then, levels hemog...

A simple analytical procedure is proposed for simultaneous determination of three common dyes (Basic Blue 9, Brilliant Blue E-4BA, and Reactive Blue 2) in natural waters without prior separation of the solutes. A popular chemometric method, partial least squares regression PLS-1, was effectively applied for spectral resolution of a highly overlapping system. At the best modeling conditions, mea...

The quantitative determination of proteins in biological fluids, using Coomassie Brilliant Blue G-250, was evaluated. Compared with the biuret method, the Coomassie Blue G-250 method needs a much shorter time for analysis and has a greater sensitivity. The sensitivity of the dye for albumin is significantly greater than for globulins. The standard curves for the biuret method are more linear th...

Hemoglobin H is of particular interest since it consists only of j3 chains (1). This abnormal hemoglobin, therefore, presents a unique opportunity for investigating the imluence of a-j3 chain interactions on some of the structural and functional properties of hemoglobin. The hemoglobin H used for these studies was obtained from two patients. The first patient, L. G., was a 25-year-old woman of ...

Cell water permeability and cell wall properties are critical to survival of plant cells during freezing, however the underlying molecular mechanisms remain elusive. Here, we report that a specifically cold-induced nuclear protein, Tolerant to Chilling and Freezing 1 (TCF1), interacts with histones H3 and H4 and associates with chromatin containing a target gene, blue-copper-binding protein (BC...