The value of CSF enzyme estimations as indices of the extent of brain damage was studied in the experimental situation. Standard cold lesions of different severity were induced in cats. The activities of the enzymes CPK, HBDH, LDH, GOT, AND ChE were studied at half hour intervals in the cerebrospinal fluid (CSF). The ventricular and cisternal fluid pressure, and the arterial blood pressure were...

Hollis Cline is the Robertson Professor of Neuroscience at Cold Spring Harbor Laboratory. She is a recent NIH Director’s Pioneer Awardee, is on the Board of Scientific Counselors for the NINDS and a previous Counselor of the Society for Neuroscience. Dr. Cline’s research investigates mechanisms of brain development using in vivo imaging, electrophysiological recordings and molecular manipulatio...

Sequential changes in the microvascular architecture of rat brains following local freezing injury were studied by scanning electron microscopy involving the cerebrovascular casting method. Repair of the microvascular architecture was initiated on day 3 after injury. Hematogenous cells and reactive astrocytes within the edematous zone participated in the regenerative process. The microvascular ...

Tissue cultures established from brain tissues of six patients with various chronic, degenerative diseases did not show evidence of viral agents. Sucrose gradient analysis of radioactively labeled culture fluid from two patients failed to reveal the presence of any incomplete or defective virus. Extracellular fluids from three brain cultures contained a factor able to stimulate deoxyribonucleic...

Hypothermia and localized cold injuries are largely preventable with proper preparation for activities in cold environments. Proficient field management is crucial to the final outcome in terms of function and viability because proper care is vital to preventing exacerbation of the initial exposure and injury. Rapid rewarming is optimal when further cold exposure can be avoided reliably. Repeti...