Staphylococcus aureus is the most prevalent and economically significant pathogen causing bovine mastitis. We isolated and characterized one staphylophage from the milk of mastitis-affected cattle and sequenced its genome. Transmission electron microscopy (TEM) observation shows that it belongs to the family Siphovirus. We announce here its complete genome sequence and report major findings fro...

Mastitis is diseases of dairy cows with a high economic impact. Bovine mastitis caused by wide range bacterial pathogens. As one the major environmental pathogens Klebsiella ssp. was investigated in this study some phenotypic characteristics like antibiotic resistance patterns and biofilm formation properties. A number 483 farms around Konya were examined California Test (CMT) producing 36 posi...

Bovine mastitis continues to be the most costly disease to the dairy farmers. It dominates in Tamilnadu as one of the most prevalent diseases in dairy cattle among the dairy farms. Mastitis treatment with antibiotics leads to the development of antibiotic resistant strains and consumer health problem. The present study is an in vitro antibacterial activity of three medicinal plants against bovi...

Fortynine quarters from 24 lactating cows with chronic mastitis were selected. The cows were raised on dairy farms in Korea. The 49 quarters with bovine mastitis were divided into control (7 quarters) and experimental (42 quarters) groups. The experimental quarters were assigned to experimental group A (10 quarters), experimental group B (14 quarters), and experimental group C (18 quarters), ac...

A total of 272 staphylococcal isolates from cases of bovine mastitis (159 Staphylococcus aureus) belonging to 12 different species were identified with ID32 STAPH galleries, and 51 of them were confirmed by 16S rRNA gene (rrs) sequencing. The same isolates were examined for their hemolytic activity on sheep blood agar, DNase activity, and coagulase activity and with two rapid identification kit...

Bovine mastitis is the most common and costly disease of dairy cattle. Cluster of differentiation 4 (CD4) is closely related to the immune response in mastitis. We quantified promoter CpG methylation levels of the CD4 gene in Chinese Holsteins with clinical mastitis (CM) and in healthy controls; these levels were quantitatively detected with bisulfite pyrosequencing assays and confirmed by clon...

The aim of this work was to identify and to characterise Staphylococcus aureus isolates associated with subclinical mastitis obtained from milk of lactating cows showing a California Mastitis Test (CMT) score result of traces, 1, 2 or 3. Coagulase, hemolysis, presence of capsule, slime formation, biofilm production, autoaggregation, hemagglutination and antibiotic susceptibility were assessed t...

Mastitis, inflammation of the mammary gland, is a common and economically important disease in dairy animals. Mammary pathogenic organisms, such as Escherichia coli, invade the teat canal,milk ducts, and mammary alveolar space, replicate in mammary secretions, and elicit a local inflammatory response characterized by massive recruitment of blood polymorphonuclear neutrophil leukocytes (PMN) int...

The bovine gastrointestinal tract is a natural reservoir for commensal and pathogenic Escherichia coli strains with the ability to cause mastitis. Here, we report the whole-genome sequences of six E. coli isolates from acute mastitis cases and six E. coli isolates from the feces of udder-healthy cows.