interferon gamma (ifn-γ) is one of the key cytokines in defining t helper 1 lymphocyte immuneresponses. in this study, the bovine ifn-γ gene was cloned from spleen tissue rna using the reversetranscription-polymerase chain reaction (rt-pcr). ifn-γ cdna was sub-cloned and expressed inmammalian expression plasmid (pcdna3.1(+)) under the control of the human cytomegalovirus (cmv)promoter. the pred...

results based on our results, mab against recombinant erns was produced and reacted successfully with recombinant and natural antigens. conclusions with regards to the role of erns in the identification of pi animals, it appears that erns recombinant antigen and the specific monoclonal antibodies produced against it may be suitable for developing bvdv laboratory diagnostic assays. objectives er...

background and aims: bovine leukaemia virus (blv) is an oncogenic member of the genus deltaretrovirus of the family retroviridae. blv is the causative agent of enzootic bovine leukaemia and infects cattle worldwide, imposing economic impact on the dairy cattle industry. the purpose of this study was to estimate the seroprevalence of blv in cattle in some provinces of iran. materials and methods...

The recent cloning and identification of a variety of regulatory and counter-regulatory molecules on T cells and antigen presenting cells has led to the development of antibodies and other molecules that either stimulate or abrogate these immune functions. Patients with autoimmune disease, graft rejection and cancer might benefit from the ability to manipulate immune regulatory pathways. The fi...

The antibody responses to human and bovine retinal S antigen in the sera of patients with uveitis from various causes were compared with those of a group of healthy volunteers who were fully screened for signs of eye disease. Antiretinal antibodies were found with equal frequency and through the same range of titres in patients and controls, irrespective of the nature or activity of the uveitis...

Two groups of patients suffering from cataract were investigated by the lymphocyte stimulation test. One group was tested before and the other after cataract surgery. Human (total) lens crystallins and bovine alpha crystallin were used as the antigens. Lymphocytes of healthy persons showed practically no positive stimulation with either antigen. Of the patients tested before operation 38% react...

Investigations were performed in lambs experimentally infected with blood lymphocytes of leukaemic cow. The presence of specific bovine leukaemia virus (BLV) antibodies in sera of infected animals was detected in agar gel immunodiffusion test and ELISA. Proviral DNA was detected with PCR and nested PCR. Dual-colour flow cytometry analysis with the use of specific monoclonal antibodies (for lymp...