Blast cells were obtained from 17 patients with acute undifferentiated leukemia and 13 patients with chronic myelogenous leukemia in blast crisis. The blasts were tested with anti-i serum in cytotoxicity tests and with antisera to myeloblastic leukemia-associated antigens in immunofluorescence tests. The terminal deoxynucleotidyl transferase (TDT) content of the blasts was also measured. Lympho...

The purposes of this study were t o examine the biologic effects of the engagement of the interleukin-7 receptor (IL-7R) with recombinant human interleukin-7 (rhlL-7) in immunophenotypically distinct T-lineage acute lymphoblastic leukemia (ALL) blasts and t o elucidate the biochemical nature of the IL-7R-linked transmembrane signal in rhlL-7responsive T-lineage ALL blast populations. In the abs...

Indoleamine 2,3-dioxygenase degrades the amino acid tryptophan which is essential for T cells. Tryptophan depletion causes T-cell cycle arrest and solid tumors that express high levels of indoleamine 2,3-dioxygenase can create immune suppression. Recently, blasts of patients with acute myeloid leukemia were shown to express indoleamine 2,3-dioxygenase. We determined INDO (encoding gene for indo...

The expression of P-glycoprotein (Pgp) is often increased in acute myeloid leukemia (AML). However, little is known of the regulation of Pgp expression by cytotoxics in AML. We examined whether Pgp expression and function in leukemic blasts was altered after a short exposure to cytotoxics. Blasts were isolated from 19 patients with AML (15 patients) or chronic myeloid leukemia in blastic transf...

a total leukocyte count (TLC) of 2.9×10/L. On day 70, he completed his second phase of induction chemotherapy and was started on G-GSF therapy for severe neutropenia (TLC: 0.76×10/L). On day 73, his peripheral blood smear showed marked leukopenia with a predominance of lymphocytes and 2% blasts without any left shift or increased granulations. This lack of prototypical G-CSF response along with...