conclusions it was the first study that attempted to detect the insertion elements isaba and ic lineages in mdr-ab species isolated from patients with burning in iran. background multidrug resistant strains of acinetobacter baumannii (mdr-ab) have emerged as alarming nosocomial pathogens among patients with burning. objectives the current study aimed to determine the susceptibility of a. bauman...

Introduction: Non-fermentative Gram-negative bacilli (NFGNB) are an important cause of healthcare-associated infections, and theyare primarily opportunistic pathogens.Aim objectives: To isolate characterize the non-fermenting from various clinical samples ina tertiary care center. detect virulence markers drug resistance using molecular methods.Material methods: were identified standardbacterio...

Acinetobacter baumannii is frequently associated with nosocomial infections, and its increasing resistance to carbapenems may significantly reduce the choice of effective antibiotics (2). Since the first description of a carbapenem-hydrolyzing class D -lactamase (CHDL), ARI-1 (renamed OXA-23), from a clinical isolate of A. baumannii in Scotland in 1995 (11), the corresponding blaOXA-23 gene has...

BACKGROUND & OBJECTIVES The nosocomial human pathogen Acinetobacter baumannii has high propensity to develop resistance to antimicrobials and to become multidrug resistant (MDR), consequently complicating the treatment. This study was carried out to investigate the presence of resistant plasmids (R-plasmids) among the clinical isolates of A. baumannii. In addition, the study was performed to ch...

background and objective : carbapenems are therapeutic choice against infections caused by gram-negative bacilli including strains of acinetobacter baumannii. resistance to these antibiotics is mediated by efflux pumps, porins, pbps and ß-lactamases. the aim of this study was to determine the possibility of existence of mbls, oxas and ges-1 betalactamase genes among clinical isolates of acineto...

OBJECTIVE To investigate the antimicrobial susceptibility and clonal relationship of Acinetobacter baumannii strains isolated in an Italian ICU. DESIGN Epidemiological, observational, retrospective, longitudinal study. SETTING AND PARTICIPANTS The ICU of the University Hospital of Sassari, Italy. MAIN OUTCOME MEASURES Pulsed Field Gel Electrophoresis (PFGE) and Multi Locus Sequence Typing...

pneumoniae ALI belonged to ST743, which is a newly identified sequence type. PCR mapping performed as described previously identified the blaOXA-48 gene that was located on the composite transposon Tn1999. Isolate ALI also possessed an intrinsic blaSHV-28 gene encoding a narrow-spectrum b-lactamase of the SHV-1 type. Plasmid location of the blaOXA-48 gene was confirmed by mating-out assays foll...

Klebsiella pneumoniae strains producing extended-spectrum β-lactamases (ESBL) exhibit resistance to antibiotic classes. The production of ESBLs (TEM-1, TEM-2, SHV-1, OXA-1) results in resistance to ampicillin, ticarcillin, piperacillin and cephalosporins. High levels of β-lactamases leads to development of resistance to β-lactamase inhibitors. The present study deals with characterizing antimic...

BACKGROUND In China, the spread and outbreak of OXA-48-producing Enterobacteriaceae remains largely unknown. METHODS OXA-48-producing isolates were analyzed for genetic relatedness by pulsed-field gel electrophoresis (PFGE), antimicrobial susceptibility by E-test, and sequence type (ST) by multilocus sequence typing. S1-PFGE and southern blotting were used for plasmid profiling, and PCR and s...