Theanine is the major amino acid present in Camellia sinensis or green tea. A method for determining theanine in its native state using liquid chromatography with positive-mode electrospray ionization mass spectrometric detection was developed. Quantitation of theanine was achieved using theanine-[(2)H(5)] as an internal standard. This approach was utilized on different green tea matrix materia...

Currently, the search for natural antimicrobial agents has increased. Thereat, plant extracts, products of fermentative processes, and microbial growth have been tested. Kombucha is a fermented beverage that resulted from fermentation infusion Camellia sinensis. There are reports about biological activities, such as anti-inflammatory, digestibility, antioxidant, antimicrobial, others. The study...

A novel form of hydroponic culture was employed to explore the physiological function of roots of a tea plant (Camellia sinensis). The pH of the nutrient solution with an actively growing tea plant decreased during cultivation. Furthermore, no oxalic acid, tartaric acid, malic acid or citric acid, all possible factors in acidification, was detected in the nutrient solution of a growing plant. A...

Tea (Camellia sinensis) is a popular beverage all over the world and a number of studies have focused on the genetic uniqueness of tea and its cultivars. However, molecular mechanisms underlying these phenomena are largely undefined. In this report, based on expression data available from public databases, we performed a series of analyses to identify genes probably relevant to the uniqueness o...

Africa is one of the key centers of global tea production. Understanding the genetic diversity and relationships of cultivars of African tea is important for future targeted breeding efforts for new crop cultivars, specialty tea processing, and to guide germplasm conservation efforts. Despite the economic importance of tea in Africa, no research work has been done so far on its genetic diversit...

Background and objectives: In recent years, phytomedicine has a main role to support immune system and in preventive medicine to decrease cancer incidence. Traditional medicine has a basic role to introduce herbal drugs to investigate in preventive medicine. Camellia Sinensis has two popular preparations, green (unfermented) and black (fully fermented) tea that among s...

Tea plant (Camellia sinensis (L.) O. Kuntze) is affected by abiotic stress during its growth and development. DNA-binding with one finger (Dof) transcription factors (TFs) play important roles in abiotic stress tolerance of plants. In this study, a total of 29 putative Dof TFs were identified based on transcriptome of tea plant, and the conserved domains and common motifs of these CsDof TFs wer...

Caffeine (1, 3, 7-trimethylxanthine) and theobromine (3, 7-dimethylxanthine) are the major purine alkaloids in plants, e.g., tea (Camellia sinensis) and coffee (Coffea arabica). Caffeine is a major component of coffee and is used widely in food and beverage industries. Most of the enzymes involved in the caffeine biosynthetic pathway have been reported previously. Here, we demonstrated the bios...

Tea plant (Camellia sinensis) leaf is an important non-alcoholic beverage resource. The application of quantitative real time polymerase chain reaction (qRT-PCR) has a profound significance for the gene expression studies of tea plant, especially when applied to tea leaf development and metabolism. In this study, nine candidate reference genes (i.e., CsACT7, CsEF-1α, CseIF-4α, CsGAPDH, CsPP2A, ...

Tea, a beverage produced by steeping processed top leaves and buds of the tea plant in freshly boiled water, originated in China 2700 BC, but its medicinal properties were know much before that. China was the first country to cultivate tea. In Japan the cultivation began about 200 AD. The use of tea later spread to the other Asian countries. Tea cultivation in India was suggested as early as 17...