Multimodality imaging has emerged as a common technological approach used in both preclinical and clinical research. Advanced techniques that combine in vivo optical and μCT imaging allow the visualization of biological phenomena in an anatomical context. These imaging modalities may be especially useful to study conditions that impact bone. In particular, orthopaedic implant infections are an ...

BACKGROUND In vivo bioluminescence imaging (BLI) is a powerful method for the analysis of host-pathogen interactions in small animal models. The commercially available bioluminescent Listeria monocytogenes strain Xen32 is commonly used to analyse immune functions in knockout mice and pathomechanisms of listeriosis. FINDINGS To analyse and image listerial dissemination after oral infection we ...

Clavibacter michiganensis subsp. michiganensis is a Gram-positive bacterium that causes wilting and cankers, leading to severe economic losses in commercial tomato production worldwide. The disease is transmitted from infected seeds to seedlings and mechanically from plant to plant during seedling production, grafting, pruning, and harvesting. Because of the lack of tools for genetic manipulati...

A significant portion of bioluminescence in all oceans is produced by dinoflagellates. Numerous studies have documented the ubiquitous distribution of bioluminescent dinoflagellates in near surface waters (Seliger et al., 1961; Yentsch and Laird 1968; Tett 1971; Tett and Kelly 1973). The number of bioluminescent species and their relative abundance change temporally, with depth, and geographica...

BACKGROUND In humans, imaging of tumors provides rapid, accurate assessment of tumor growth and location. In laboratory animals, however, the imaging of spontaneously occurring tumors continues to pose many technical and logistical problems. Recently a mouse model was generated in which a chimeric protein consisting of HIF-1α oxygen-dependent degradation domain (ODD) fused to luciferase was ubi...

The use of genetically engineered bioluminescent bacteria, in which bioluminescence is induced by different modes of toxic action, represents an alternative to acute toxicity tests using living aquatic organisms (plants, vertebrates, or invertebrates) in an aqueous environment. A number of these bacterial strains have been developed, but there have been no attempts to develop a hand-held type o...

This video describes the establishment of liver metastases in a mouse model that can be subsequently analysed by bioluminescent imaging. Tumour cells are administered specifically to the liver to induce a localised liver tumour, via mobilisation of the spleen and splitting into two, leaving intact the vascular pedicle for each half of the spleen. Lewis lung carcinoma cells that constitutively e...

Advances in chemical design, genetic engineering, and biomedical technologies have contributed to impressive progress in bioluminescence imaging (BLI) of complex biological systems from cells to living mammals. While the technique is prevalent in small animal in vivo molecular imaging applications, most tools have been limited to only a few sets of bioluminescent systems. Now, the Prescher lab ...