Flocculants agglomerate suspended microalgae cells, while cost and toxicity have led to the increased use of bio-flocculants. In this experiment, Chlorella pyrenoidosa was gathered by utilizing bio-flocculants from discarded clam shells. At pH 8, 0.2 mg/mL bio-flocculant shell, 0.1 cationic inducer, 240 rpm mixing achieved 91.87 % flocculation efficiency 458.1 mg recovered biomass. Calcium ions...