Since its inception by Svedberg and coworkers in the 1920s, the analytical ultracentrifuge has provided a powerful tool in biochemistry and molecular biology, with applications ranging from simple purity checks and particle shape determinations from sedimentation velocity, isolation and purification of macromolecules using density gradient techniques right through to the evaluation of mol wt an...

Oligomerization of connexins is a critical step in gap junction channel formation. Some members of the connexin family can oligomerize with other members and form functional heteromeric hemichannels [e.g. Cx43 (connexin 43) and Cx45], but others are incompatible (e.g. Cx43 and Cx26). To find connexin domains important for oligomerization, we constructed chimaeras between Cx43 and Cx26 and studi...

BACKGROUND Primary sclerosing cholangitis is a rare chronic cholestatic condition of unknown etiology, frequently associated with inflammatory bowel disease and characterized by diffuse fibrosing and inflammatory destruction of the intra- and/or extrahepatic biliary duct system. PATIENTS AND METHODS The study involved 14 children with primary sclerosing cholangitis confirmed by either liver b...

BACKGROUND/AIM Every surgical procedure causes metabolic, endocrine, and hemodynamic stress in the organism. The aim of this work was to assess the extent of trauma following each of the two types of cholecystectomy (traditional/open and laparoscopic) by measuring palette of biochemical parameters. METHODS This prospective, single- center study included 120 patients subjected to elective chol...

Gravitropism is vital for shaping directional plant growth in response to the forces of gravity. Signals perceived in the gravity-sensing cells can be converted into biochemical signals and transmitted. Sedimentation of amyloplasts in the columella cells triggers asymmetric auxin redistribution in root tips, leading to downward root growth. The actin cytoskeleton is thought to play an important...

The Hermansky-Pudlak syndrome defines a group of genetic disorders characterized by defective lysosome-related organelles such as melanosomes and platelet dense bodies. Hermansky-Pudlak syndrome can be caused by mutations of at least four genes in humans and 15 genes in mice. One of these genes is mutated in the pallid mouse strain and encodes a novel protein named pallidin (L. Huang, Y. M. Kuo...

Deposition of sediment inside the conduit structures reduces the flow cross section area and increases the flow roughness which causes reduction of flow discharge passing through the conduit. Among the methods which can prevent sediment deposition is non sedimentation condition in the structure, Since the critical section of the inverted siphon in sedimentation is the outlet. In the begining of...

The zeta sedimentation ratio (ZSR) capillary tube, is subjected to four is a measurement similar to the detercycles of dispersion and compaction. mination of the erythrocyte sedimenAt the conclusion of this process, the tation rate (ESR) but possesses sevaral hematocrit of the blood in the red advantages. It is unaffected by anemia cell-containing portion of the capillary and responds in a line...

(b) Sedimentation equilibrium: an experiment performed at a lower speed so that the sedimentation and backdiffirsion forces are of comparable magnitudes and therefore give rise to an equilibrium distribution of solute concentration. Because there is no net transport at equilibrium, shape effects do not come into play and the distribution becomes an absolute function of molecular mass for a sing...

We present the validation of a recent fractional mathematical model for erythrocyte sedimentation proposed by Sharma et al. \cite{GMR}. The model uses a Caputo fractional derivative to build a time fractional diffusion equation suitable to predict blood sedimentation rates. This validation was carried out by means of erythrocyte sedimentation tests in laboratory. Data on sedimentation rates (pe...