In the era of the Human Genome Project, quantitation of gene expression by tumor/host cells is of paramount importance to investigate gene patterns responsible for cancer development, progression and response/resistance to treatment. Quantitative real-time PCR (qrt-PCR) technology has recently reached a level of sensitivity, accuracy and practical ease that support its use as a routine bioinstr...

PCR primer design is an everyday, but not trivial task requiring state-of-the-art software. We describe the popular tool GeneFisher and explain its recent restructuring using workflow techniques. We apply a service-oriented approach to model and implement GeneFisher-P, a process-based version of the GeneFisher web application, as a part of the Bio-jETI platform for service modelling and executi...

The development of miniaturised devices for genetic diagnosis is of great interest for medicine applications. In this paper, we focus on the design, the experimental technology and characterization of a new silicon micro-bio-chip for rapid testing of DNA material. This chip integrates two classical processes for DNA analysis: polymerase chain reaction – PCR – technique for fragments amplificati...

INTRODUCTION Low or undetectable plasma viral load (VL) using current qPCR assays is common for HIV-2 patients. Digital PCR is an emerging technology enabling more precision and reproducibility than qPCR at low DNA/RNA copy numbers. Available data related to digital droplet PCR (ddPCR, Bio-Rad) underscore issues linked to the threshold definition of positivity, coupled to the specificity of low...

in order to study the effects of integrated application of bio-fertilizer and chemical fertilizers on yield and yield components of corn, an experiment was conducted in 2008 in dezful city, (khoozestan province) in a factorial arrangement based on complete randomized block design with four replications. treatments were integrated application of biological and chemical fertilizers in four levels...

background: basil (ocimum basilicum l.), a member of the lamiaceae family, has traditionally been used as a medicinal plant in the treatment of headaches, coughs, diarrhea, constipation, warts, worms, and kidney malfunctions. objective: to investigate the foliar application effects of bio-stimulators and bio-fertilizers on morphological and phytochemical traits of basil (ocimum basilicum l.). m...

objective: the aim of present study was to evaluate the quality, density and thickness of newly formed bone in experimental defects treated with combi-pack®, bio-oss® and biostite®. materials and methods: eight new zealand white rabbits were included in this randomized,blinded study. four equal 3×6 mm bone defects were created on the frontal and parietal bones of each animal and three were imme...

the co-inoculation of mulberry with phosphate solubilizing micro-organisms, nitrogen fixing bacteria and arbuscular mycorrhiza has influenced its macronutrient uptake through leaf. the data revealed that maximum nitrogen (484.12 kg /ha) , phosphorus (59.83 kg /ha) and potassium (244.61 kg/ha) uptake through leaf has taken place due to co-inoculation treatments as compared to the un inoculated t...

background: bio aerosols include airborne micro-organisms such as bacteria, fungi, viruses, etc and their products. exposure to a bio aerosol is linked with a broad spectrum of health problems, including infectious diseases, acute toxic effects, allergies, and cancer. the aim of this study was to evaluate the quality and quantity of bio aerosols found in the air of different wards in valiasr ho...