The kinetic rate equation for the photosensitized cleavage reaction of surface-bound photolabile chromophores with free diffusion of sensitizer molecules from the bulk of a solution to the surface is derived by determining the stationary solution of a diffusion equation with suitable boundary conditions. The relation between the phenomenological rate constant for the photosensitized reaction at...

The endocytotic rate constant (k,) is a cellular constant which defines the probability of an occupied receptor being internalized in 1 min at 37 "C. We have derived an equation that expresses the relationship between k, and the relative amount of surface-bound and internalized ligand as a function of time. This equation was used as the basis for a graphical form of analysis called the In/Sur p...

Apotransferrin (apo Tf) in 0.1 M N-(2hydroxyethyl)piperazine-N2-ethanesulfanic acid at 25 ̊C and pH 7.4 has been titrated with acidic solution of Tb. The binding of Tb at the two specific metal-binding sites of transferrin was followed from the changes in the difference UV spectra at 245 nm. The molar absorptivity per binding site for Tb-Tf is 22,500 ± 1000 Mcm. To determine the Tb-Tf binding c...

We apply our tight-binding (TB) methodology to the sp metals Al, Ga and In, all of which have distinctive ground states. The results show that this approach works as well for such elements as it does for transition metals. Bulk properties such as lattice constants, bulk moduli and elastic constants were found to be consistent with experiments. We emphasize that our method successfully predicts ...

ClC-0 is a chloride channel whose gating is sensitive to both voltage and chloride. Based on analysis of gating kinetics using single-channel recordings, a five-state model was proposed to describe the dependence of ClC-0 fast-gate opening on voltage and external chloride (Chen, T.-Y., and C. Miller. 1996. J. Gen. Physiol. 108:237-250). We aimed to use this five-state model as a starting point ...

The Caulobacter crescentus CgtA protein is a member of the Obg-GTP1 subfamily of monomeric GTP-binding proteins. In vitro, CgtA specifically bound GTP and GDP but not GMP or ATP. CgtA bound GTP and GDP with moderate affinity at 30 degrees C and displayed equilibrium binding constants of 1.2 and 0.5 microM, respectively, in the presence of Mg(2+). In the absence of Mg(2+), the affinity of CgtA f...

When commitment schemes are used in complex environments, e.g., the Internet, the issue of malleability appears, i.e., a concurrent man-in-the-middle adversary might generate commitments to values related to ones committed to by honest players. In the plain model, the current best solution towards resolving this problem in a constant number of rounds is the work of Ostrovsky, Persiano and Visco...

Intentional binding describes the phenomenon that actions and their effects are perceived to be temporally approximated. We introduced a new method of duration estimation to the research field, the method of constant stimuli. Participants freely chose to press one of two keys or experienced passive key presses. After an interval of 250 ms or 600 ms a visual effect occurred. In Experiment 1, eac...