The reaction of NiCl2, K2(i-mnt) (i-mnt2−= 2,2-dicyanoethylene-1,1-dithiolate) and 1-benzyl-4-amino-pyridinium bromide [BzPyNH2]Br affords a nickel complex, [BzPyNH2]2[Ni(i-mnt)2], molecular cata...

Three acyl derivatives of amino acids, dicarbobenzoxy-l-lysine sodium, carbobenzoxy-l-aspartic acid-beta-benzyl ester potassium, and N-3-phenylpropionyl-S-benzyl-l-cysteine potassium inhibit the growth of parainfluenza 1 (hemadsorption 2) virus. The growth of simian virus 40, vaccinia, poliomyelitis type 1, Semliki Forest, Eastern equine encephalitis, and Western equine encephalitis viruses was...

Selected 2,6-(disubstituted)purine 2',3'-didehydro-2',3'-dideoxynucleosides and 2',3'-dideoxynucleosides were prepared and evaluated. Treatment of 5'-protected ribonucleosides with phenoxythiocarbonyl chloride and 4-(dimethylamino)pyridine, or under Schotten-Baumann conditions, gave high yields of 2',3'-O-thionocarbonates that underwent Corey-Winter elimination. Treatment of unprotected ribonuc...

In brief: Metabolite signatures were compared between children with normal weight, obesity, and both obesity T2DM, by measuring 273 analytes in fasting plasma a 24-hour urine sample. Twenty-two metabolites uniquely associated T2DM. Adolescents T2DM have altered purine nucleotide metabolism, betaine oxidative branched-chain amino acids (BCAAs) their catabolites.

Normal human lymphoblasts starved for each of several essential, but not nonessential, amino acids had decreased DNA and RNA synthesis but no change in free intracellular purine nucleotides. The rates of purine nucleotide synthesis via the de novo and salvage pathways were measured by incorporating [14C]formate and [“C]hypoxanthine labels, respectively, into lymphoblasts starved for an amino ac...

Base composition, codon usages and amino acid usages have been analyzed by taking 529 orthologous sequences of Aquifex aeolicus and Bacillus subtilis, having different optimal growth temperatures. These two bacteria do not have significant difference in overall GC composition, but GC(1+2) and GC3 levels were found to vary significantly. Significant increments in purine content and GC3 compositi...

Gaffkya homari, a micrococcus isolated from septicemic lobsters, has an obligate purine requirement (Aaronson, 1956). Further study shows this requirement to be satisfied by several natural purines as well as by several purine analogues. This broad response suggested that G. homari might be useful for studying purine biosynthesis. We wished especially to know whether G. homari utilized 4-amino-...

In order to clarify the role of the purine 2-amino group in the recognition of DNA by small molecules we have examined the binding of actinomycin D and echinomycin to artificial DNA molecules asymmetrically substituted with inosine and/or 2,6-diaminopurine (DAP) in one of the complementary strands. These DNAs, prepared by a method based upon PCR, present various potential sites for antibiotic b...

6-(p-Hydroxyphenylhydrazino)uracil (H2-HPUra) is a selective and potent inhibitor of the replication-specific class III DNA polymerase (pol III) of Gr+ bacteria. Although formally a pyrimidine, H2-HPUra derives its inhibitory activity from its specific capacity to mimic the purine nucleotide, dGTP. We describe the successful conversion of the H2-HPUra inhibitor prototype to a bona fide purine, ...