The complete nucleotide sequence of RNA 3 of the Ahlum isolate of beet soil-borne virus (BSBV) was determined from cDNAs obtained with immunocaptured virus particles and denatured preparations of dsRNA. BSBV RNA 3 is unique among the plant virus RNAs studied so far in containing apparently only the coding sequences of a triple gene block (TGB). The derived amino acid sequences of the three puta...

Background: Polymyxa betae is the vector of several soil-borne viruses of sugarbeet (Abe and Tamada 1986, Liu and Lewellen 2008, Wisler et al. 1994, Kaufmann et al. 1992), including Beet necrotic yellow vein virus (BNYVV), the cause of rhizomania (Tamada and Baba 1973). BNYVV is transmitted by zoospores of this fungus-related microorganism that swim in water films and infect feeder roots of sug...

To study molecular evolutionary characteristics and genetics of beet necrotic yellow vein virus (BNYVV) isolates population from Iran, nucleotide sequences of p25 and coat protein (CP) were determined and the amino acids sequences thus deduced were analyzed using phylogenetic and population genetics methods. A survey of BNYVV in Iran indicated the infection of 288 collected samples out of 392 s...

A new gene-expression system based on RNA-5 of Beet necrotic yellow vein virus (BNYVV) was constructed to allow the expression of recombinant proteins in virally infected cells. Replication and expression levels of the RNA-5-based replicon containing the green fluorescence protein (GFP) gene were compared with those obtained with the well-characterized RNA-3-derived replicon (Rep-3). When RNA-3...